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Objective: the application Qilan particles through the establishment of the rat tongue mucosa cancer model and to intervene to observe the resistance of cancer tumor suppressor role, while using immunohistochemical SABC method of proliferating cell nuclear antigen (PCNA), epidermal growth factor receptor (EGFR), p27 protein expression. Possible mechanism to explore of particles blocking cancer Qilan tumor suppressor. Methods: Animals modeling choice of 6 to 7-week-old SD rats were 150 randomly divided into five groups (30 rats / group), group A carcinogenesis model group, B group for the low-dose group, C group in dose intervention group D group intervention for the high-dose group, E group for the normal control group. Drinking Water Act administration, were given 0.002% 4NQO and 0.002% 4NQO low, middle and high dose Qilan particles carcinogenesis model in rats and drug intervention model. 9 weeks, 18 weeks, 27 weeks, 36 weeks sacrificed animals (7 / week), take the tongue tissue for biopsy, immunohistochemistry (SABC) PCNA, EGFR, p27 protein expression and statistics software for statistical analysis of the experimental results. Results: 1. Qilan particles have the effect of righting and tumor suppressor resistance cancer: A group of rats with respect to B, C, D rats fatigue lethargy, fur color yellow, rough and dull, gradual weight loss; 27, 36 weeks, Groups A, B, C, D cancerous degree of difference was statistically significant, the overall rate of cancer than rats A B C D group higher (p lt; 0.05), where C group the lowest level of cancer (p lt; 0.01) (2) from normal oral mucosa → levels of dysplasia → squamous cell carcinoma lesions, PCNA, EGFR protein expression showed an increasing trend (p lt; 0.05), p27 protein expression showed a decreasing trend (p lt; 0.05). Abnormal proliferation of tongue tissue in group A, PCNA and EGFR protein expression was significantly higher than the Chinese intervention group (p lt; 0.05), p27 expression was significantly lower than in group A Chinese intervention group (p lt; 0.05); A group of PCNA and p27 protein expression with traditional Chinese medicine intervention group between tongue cancer was no significant difference (p gt; 0.05), EGFR protein expression was significantly higher than that in group A Chinese intervention group (p lt; 0.05 ). 4 in the abnormal proliferation of tissue expression of PCNA and p27 protein expression was negatively correlated (r = -0.786, p lt; 0.001), PCNA protein expression and EGFR protein expression was positively correlated (r = 0.588, p lt; 0.001); cancerous tissue expression of PCNA protein expression of P27 protein was negatively correlated (r = -0.502, p lt; 0.05), EGFR protein associated with cell proliferation activity unrelated (r = 0.252, p gt; 0.05). Conclusions: 1 Qilan particles have a righting effect. 2 Qilan particles can significantly reduce the incidence of dysplasia and cancer of the tongue mucosa, slow tongue carcinogenesis process, experimental oral carcinogenesis has obvious resistance cancer, a tumor suppressor role. 3, PCNA, EGFR and p27 expression in oral squamous cell carcinoma occurrence and development, can be used as a marker for evaluation of oral squamous cell carcinoma cell proliferation. 4, dysplasia stage, EGFR can promote cell proliferation, p27 inhibition of cell proliferation, both mutual restraint regulation in tumor formation; malignant tumor stage, p27 is still limiting the progress of cancer. 5, Qilan particle resistance mechanisms of cancer may be dysplasia stage by down-regulating the expression of PCNA and EGFR protein, and at the same time increase the expression of p27 protein, thereby inhibiting the abnormal proliferation of epithelial cells, play a role in blocking cancer, and cancer organization through down the expression of EGFR play a role in inhibiting cancer.
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