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Study of Screening Conditions of the Zeolite Absorption on Bacillus Natto and B.Natto Relieving the Negative Impact on Broilers Caused by Clostridium Perfringens

Author: SunYaZuo
Tutor: WangTian；ZhouYanMin
School: Nanjing Agricultural College
Course: Animal Nutrition and Feed Science
Keywords: Bacillus natto zeolite Clostridium Perfringens performance serum markers apoptosis
CLC: S831
Type: Master's thesis
Year: 2009
Downloads: 50
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Abstract


By taken zeolite as the adsorbent screened the adsorption condition of Bacillus natto, and to study the effect of B.natto on broilers after Clostridium Perfringens infection. This study included three parts and their results were presented as follows:The growth curve of B.natto and amount of ammonium absorption of zeolite before and after modification were determined in the first experiment. Plackett-Burman experimental design and complete factorial design were used to study the optimum condition of zeolite adsorbing B.natto. The result showed:The optimum culture time of B.natto was 8h; the amount of ammonium absorption of zeolite increased with the decreased particle size, high temperature modification could increase the amount of ammonium absorption of zeolite. The optimum condition of zeolite adsorbing B.natto was: particle size 400 mesh, addition 0.7g/mL, time lOmin, temperature 35℡℃speed 150rpm.The second experiment studied the effect of B.natto on performance and related serum markers of broilers after Clostridium Perfringens infection.480 AA broiler chicks were randomly to 5 treatments, of 16 pens for 6 replicates. The details for 5 treatments were described as follows:Control (non-infection) group with basal diet, infection group (Clostridium Perfringens infection) with basal diet, antibiotic group(Clostridium Perfringens infection) with basal diet added 400mg/kg zinc bacitracin, 100mg/kg B.natto group(Clostridium Perfringens infection) with basal diet added 100mg/kg B.natto, 200mg/kg B.natto group(Clostridium Perfringens infection) with basal diet added 200mg/kg B.natto. Broilers expect Control group was infected by low dose Clostridium Perfringens in 14-16d and 34-36d, and be determined on performance and related serum markers. The result showed:1. Clostridium Perfringens infection could decrease broilers’ performance; serum MDA increased 30.56%(P>0.05) in 21d and 95.60%(P<0.01) in 42d, serum SOD decreased 29.74(P<0.01) in 21d and 5.90%(P>0.05) in 42d, serum GSH-Px decreased 20.38%(P<0.05) in 21d and 16.82%(P>0.05) in 42d; serum AKP increased 61.07%(P<0.05) in 42d; serum T3 and T4 had no significant effect(P>0.05), but serum T3 was increased in a certain degree; serum IgA and IgG was increased(.P>0.05) in a certain degree in 21d and decreased(P>0.05) in a certain degree in 42d.2. Compared to the infection group, added 200mg/kg B.natto could improve broilers’ performance during the later and whole trial period.3. Compared to the infection group, added 100mg/kg B.natto could decrease serum MDA 18.18%(P>0.05) in 21d and 28.61%(P<0.01) in 42d, added 200mg/kg B.natto could decrease serum MDA 19.01%(P>0.05) in 21d and 29.40%(P<0.01) in 42d; added 100mg/kg and 200mg/kg B.natto could increase serum SOD 20.05%(P>0.05) and 20.11%(P>0.05) in 21d; added B.natto could increase serum GSH-Px in a certain degree, but had no significant effect(P>0.05).4. Compared to the infection group, added 100mg/kg and 200mg/kg B.natto had no significant effect(P>0.05) on biochemical parameters, but serum AKP decreased 14.38% and 17.70%(P>0.05).5. Compared to the infection group, B.natto significantly increased serum T4(P<0.05) in 42d, but had no significant effect on serum T4 in 21d(P<0.05) and serum T3 in 21d(P<0.05) and 42d(P<0.05).The third experiment studied the effect of B.natto on organ index, serum DAO, serum NO and cell apoptosis of broilers after Clostridium Perfringens infection. The experiment materials and methods were as same as that in the second experiment. The results showed: 1. Compared to the control group, the organ index of broilers had a certain increasing after Clostridium Perfringens infection, but had no significant effect(P<0.05); Clostridium Perfringens infection could significantly increased serum DAO 37.68%(P<0.05), serum NO 138.61%(P<0.01) in 21d and 14.37%(P>0.05) in 42d; broilers’ thymus death cells were increased(P>0.05) and living cells were decreased(P>0.05) after Clostridium Perfringens infection.2. Compared to the infection group, serum DAO decreased 17.73%(P>0.05) and 19.61%(P>0.05) after added 100mg/kg and 200mg/kg B.natto.3. Compared to the infection group, added 100mg/kg and 200mg/kg B.natto could decreased serum NO 29.93%(P<0.01) and 48.51%(P<0.01) in 21d,7.27%(P>0.05) and 10.99%(P>0.05) in 42d.4. Compared to the infection group, added B.natto could decrease thymus death cells(P>0.05) and increase living cells(P>0.05) in 42d, decrease thymus apoptosis cells(P>0.05) in 21d; B.natto had no significantly on spleen cells(P>0.05); B.natto decreased intestine mucosa apoptosis cells(P>0.05) in 21d and death cells(P>0.05) in 42d, increased living cells(P>0.05) in 42d.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Poultry > Chicken
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