Dissertation > Excellent graduate degree dissertation topics show

Effects of Melatonin on Cultured Rat Müller Cells under High Glucose Conditions

Author: YanSaiMei
Tutor: ChangQing;HuangXin;WangLing
School: Fudan University
Course: Ophthalmology
Keywords: Müller cell Diabetic retinopathy Melatonin Nrf-2 HO-1
CLC: R587.2
Type: Master's thesis
Year: 2009
Downloads: 21
Quote: 0
Read: Download Dissertation

Abstract


Purpose:The present study was designed to elucidate the antioxidant effects of melatonin on rat M(u|¨)ller cell cultured in vitro with high glucose and to examine whether melatonin receptor MT1/MT2 would be expressed and involved in melatonin-mediated cytoprotection of rat M(u|¨)ller cell.Methods:(1) Rat M(u|¨)ller cell was cultured in Dulbecco modified Eagle medium supplemented with 20%fetal calf serum.(2) The cells were divided into control group,high glucose group,mannitol group,The expression of the MT1,MT2(melatonin receptor) were examined with retroverse-transcriptinal polymerase chain reaction analysis.(3)The cells were divided into normal glucose group,mannitol group,normal glucose group+melatonin group,high glucose group,high glucose plus melatonin and high glucose plus melatonin plus Luzindole group.The expression of Nrf-2 and HO-1 were examined with retroverse-transcriptinal polymerase chain reaction analysis.Results:Immunocytochemical studies demonstrated that GS(glutamine synthetase) is expressed in almost all M(u|¨)ller cells.RT-PCR analysis revealed similar expression levels of MT1 and MT2,and after cultured in high glucose medium for 48 hours the expression of MT1 and MT2 were both quadrupled.An examination of the effects of melatonin on antioxidant expression revealed that low pharmacological concentrations of this hormone caused a significant induction of HO-1 mRNA and Nrf-2 expression in M(u|¨)ller cells following treatment for 48 hours,the expression can be neuralized by its receptor antagonist-Luzindole.Conclusions:we provide the first evidence that melatonin induced increased expression of Nrf-2 and HO-1 and might have a beneficial effect in protecting against glial cell dysfunction by high glucose-induced oxidative stress in vitro.

Related Dissertations

  1. Study on the Green Synthesis of Melatonin,R914
  2. Cross-Talk between Ethylene and Heme-Heme Oxygenase Signal System in Primary Root Growth Inhibition of Etiolated Tomato Seedlings,S641.2
  3. Study on Therapy and Mechanisms of the Role of Tang Kang in Diabetic Rat and Endothelial Cells,R285.5
  4. Diabetic retinopathy OK vitrectomy surgery intraocular lens implantation in different periods of clinical observation,R779.66
  5. The Effect of Melatonin on Calcium Overload of Fetal Mouse Brain Cells Due to Lipopolysaccharide,R722.1
  6. Effect of Quercetin/hemeoxygenase-1/bilirubin System on Ethanol-induced Injury of Rat Hepatocytes,R285.5
  7. Study on the Different Expression of Heme Oxygenase Enzyme in Human Testis of Fertile and Infertile Males,R698.2
  8. Effects of Melatonin on TGF-β3 Level in Hippocampus of Rats with Seizures Induced by Kainic Acid,R742.1
  9. pEGFP/Ang-1 Transfected Rat BMSCs Transplantation on Diabetic Retinal Vascular Leakage of Retinal,R774.1
  10. Theoretical and Clinical Study on Treatment of Diabetic Retinopathy Based on the Collateral Theory,R259
  11. Study on the Upconversion White Light Properties of Ho: Yb: Tm: LiNbO3 Polycrystals,O482.31
  12. Sanmen County , Zhejiang Province, rice production in Ho Preliminary Study on Ecological Regulation,S511
  13. Structure Change of Macular Arch in Diabetic Retinopathy,R774.1
  14. Severe Proliferative Diabetic Retinopathy Treated by Pars Plana Vitrectomy Assisted by Medicine Intravitreal Injection,R779.6
  15. Ang-1 and VEGF Expression in Early Diabetic Retinopathy in Rats,R774.1
  16. The Study of Mechanisms of Myopia on Preventing Diabetic Retinophty,R587.2
  17. Laboratory diagnosis of depression - circadian rhythm of biological markers,R749.4
  18. Investigation of Retinal Nerve Fiber Layer Changes in Different Stage of Type Ⅱ Diabetes Mellitus,R587.1
  19. Diabetic nephropathy in patients with type 2 diabetes and diabetic retinopathy study,R587.2
  20. Constructing Evaluation System for Protein Nitration and Inhibition,R587.1
  21. Type 2 diabetic retinopathy and serum retinol binding protein 4 levels of correlation,R587.2

CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetic coma and other complications
© 2012 www.DissertationTopic.Net  Mobile