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h-VEGF165 Genetransfer Promoted the Tissue Repair of Maxillofacial Region in Rat

Author: ShenZongZe
Tutor: ZhengJianJin；JiaMuYun
School: Qingdao University
Course: Clinical Stomatology
Keywords: VEGF165 TGF-β1 recombinant plasmid Lipofectamine radiation injury rat
CLC: R730.5
Type: Master's thesis
Year: 2011
Downloads: 19
Quote: 0
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Abstract


Objective:This study was using genetransfer way that treated some disease which had noneffective form routine drugs or traditional therapeutics. And then understand how the effective of VEGF165 plasmid gene expression in tissue at after genetransfer. At the same time we observed pathological change of masseter injury of radiated rats and the level of TGF-β1 gene expression.Methods:1.We used the human VEGF165 cDNA in patients of oophoroma peripheral blood was amplified using RT-PCR, and then the recombinant plasmid was constructed after cutting VEGF165 and pcDNA4-HisMax-C respectively by restriction enzyme BamHI and BamHI. And then the way of gene clone by delivered the pcDNA4-HisMax-C/VEGF165 into Escherichia coli. Eventually, the successful construction plasmid was comfirmed by the identification of endonuclease cutting, sequencing.2. Constructed the Animal Model of irradiation injury, all animal experiments irradiation dose of 40 Gy, radioactive source was used linear accelerator, and observed the pathological change of vessel under light microscope and electron microscope and detected the expression of TGF-β1 by RT-PCR.Results:We were successful construction VEGF165 form oophoroma patients and constructed the pcDNA4-HisMax-C/VEGF165-Second, the microvessel density of control group was higher than that of radiated group significantly. The expression of TGF-β1 in radiated group was higher than that in control group significantly. When after the VEGF165 gene transfer, the rat of VEGF-A expression was different with gene transfer before.Conclusion:We used the irradiation dose of 40 Gy by linear accelerator radioactive source on rat and successful to simulate the pathological change of patients’irradiation injury. on the other hand, a new pcDNA4-HisMax-C/VEGF165 could provided an efficent tool for radioactive vascular endothelial injury.

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