Dissertation > Excellent graduate degree dissertation topics show

Hyperuricemia right OLETF and LETO rat aortic smooth muscle cells ki-67 and URAT1 expression

Author: SongFei
Tutor: ZhaoWenJuan
School: Qingdao University
Course: Endocrine and metabolic diseases
Keywords: Hyperuricemia Multiplication of vascular smooth muscle cell Ki-67 URAT1 transporter OLETF rat
CLC: R589.7
Type: Master's thesis
Year: 2011
Downloads: 31
Quote: 0
Read: Download Dissertation

Abstract


OBJECTIVE:To observe the effect of hyperuricemia on expression of Ki-67 and URAT1 of rat’s aortic smooth muscle cells(ASMC). METHODS:20 male OLETF rats of 4 weeks age were raised up to 25 weeks when the rats were suffering from hyperinsulinemia、disturbance of lipid metabolism and adiposity, then were divided randomly into control group (n=10)and high purine diet group (n=10).The high purine diet group was administered with adenine (50mg/kg/d) by gavage and was fed ground standard rat chow containing 10% yeast.20 male LETO rats of 4 weeks age were processed the same way with OLETF.TG,TC,UA,CRE,BUN and 24h urine volume and UUA were detected. Insulin were detected by radioimmunity. The incidence rate of hyperuricemia was compared between the OLETF and LETO rats. Kill all of the rats after three weeks of high purine diet. The samples of thoracic aorta from rats were sliced into the paraffin sections. HE staining was used to observe the aortic smooth muscle structure and immunohistochemistry to detect the expression of Ki-67 and URAT1. RESULTS:1.①On the 11th day of high purine diet, blood uric acid level of both OLTEF and LETO high purine diet group were significantly higher than their corresponding control group (P=0.000). The hyperuricemia model of rats was formed successfully.;②The incidence rate of hyeruricemia of OLTEF high purine diet group (80%) was significantly higher than that of LETO high purine diet group(30%)(P<0.05).Moreover, blood uric acid level of OLTEF high purine diet group was much higher than that of LETO high purine diet group (P=0.00).That was to say the OLETF rats suffering from Reaven syndrome were more likely to develop hyperuricemia;③All of the rats showed no obvious signs of thoracic aortic atherosclerosis nor foam cells, so there were no statisticl significance between LETO and OLETF rats (P>0.05).④. No expression of Ki-67 and URAT1 was showed that there were also no statisticl significance between the two groups (P>0.05).⑤The expression of URAT1 was detected on fat cells. CONCLUSION:①High purine diet persisted for 3 weeks had no obvious effect on the expression of Ki-67 and URAT1 on 28 weeks old male rats, either LETO or OLETF, nor result in atherosclerosis. As one of the factors of atherosclerosis, it need more time for hyperuricemia to result in atherosclerosis.②Fat cell expresses URAT1 and that maybe have relevance with hyperlipemia、fat and hyperinsulinism etcl, but it should be further attested through more study.

Related Dissertations

  1. The Expression of MMP-2、 VEGF and Ki-67 and Human Glioma Grade,R739.41
  2. The Relationship between CEF/TE Neo-adjuvant Chemotherapy, MAPK/PI3K Signal Pathway and Ki-67 in Breast Cancer,R737.9
  3. Protein Expressions of EGFR、K-ras、P53 and Ki-67 in Colorectal Cancer, Gastric Cancer and Esophageal Cancer,R735
  4. The Expression and Clinical Significance of Her-2/Neu and Ki-67 in Gastric Carcinoma,R735.2
  5. Relationship between Expression of COX-2 and Cell Cycle Regulatory Proteins in Patients with Esophageal Squamous Cell Carcinoma,R735.1
  6. Ⅰ, Ⅱ type psoriasis clinical analysis and ERβ in its expression and significance of lesions,R758.63
  7. SLC2A9 gene eighth exon 137A / G polymorphism and diabetic patients with hyperuricemia relationship,R587.1
  8. Ki-67 and Bc1-2 in oral squamous cell carcinoma and its significance,R739.8
  9. The Expression of Cortistatin in Psoriasis and the Effect of Exogenous Cortistation on a Proliferation Model of EGF-induced HaCaT Cell,R758.63
  10. Correlative Research of Expression of ERCC1,BRCA1,Ki-67 and Outcome of CEF Neoadjuvant Chemotherapy of Breast Cancer,R737.9
  11. The Significance of P16INK4a,Ki-67 and Telomerase Expression in Squamous Epithelial Lesion of Cervix,R737.33
  12. The Expression of IgA in EIN and Esophageal Cancer,R735.1
  13. Kazak hyperuricemia and relationship between glucose and lipid metabolism,R589
  14. Effects of Rosuvastatin Calcium on CRP, IL-6 and TNF-α in Hyperuricemia Rats Model,R589.7
  15. Bcl-xL and Fas Protein Change in Spermatogenic Cells of OLETF Rat Testes and Rosiglitazone Intervention Effect,R587.1
  16. Correlation of VEGF-C, K-i67 and C-erbB-2 Expression in Breast Cancer and their Clinical Significance,R737.9
  17. Human Papilloma Virus 16/18 Infection in Head and Neck Squamous Cell Carcinoma: Incidence, Possible Mechanism and Prognosis,R739.91
  18. The Significance of COX-2 and Ki-67 Expressing in Hepatobiliary Calculus with Cholangiocarcinoma,R735.8
  19. The Study of Correlation of CT Features, Pathology and Ki-67, p53 , Serum Tumor Markers in Peripheral Lung Cancer,R734.2
  20. Expression and Clinical Significance of Annexin A2, NF-κBp65 and Ki-67 in Cervical Cancer,R737.33
  21. Clinicopathologic Significance of SIRT1 and p300/CBP Expression in Gastric Cardia Carcinoma,R735.2

CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Metabolic diseases > Metabolic disorder of purine ( Purine )
© 2012 www.DissertationTopic.Net  Mobile