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In our study, the expression patterns and the methylation analysis of the promoter of 2810025M15Rik were reported. The expression patterns were characterized by real-time quantitative RT-PCR and in situ hybridization; the methylation analysis of the CpG island was detected with bisulfite specific PCR(BSP); Also, direct sequencing of the PCR products was carried on to identify whether this gene is an imprint gene. Whole embryo in situ hybridization results showed that the expression signals were found in the telencephalon, midbrain, hindbrain, branchial arch, forelimb bud and tail bud of E10.5 and E11.5 mouse embryos, signals were also detected in the rhombencephalon and hindlimb buds in E11.5 mouse embryos. Section in situ hybridization of E13.5 showed strong expression signals in the forebrain, midbrain, third ventricle of the cerebral cortex, as well as the liver. Results of real-time quantitative RT-PCR revealed that there was a relatively high transcriptional level of 2810025M15Rik in E12.5 mouse embryos; at the stage of E15.5, the expression level of this gene was strongest in lungs, then followed by in brain and kidneys, weakest in liver; At E18.5, the expression level had significant changes in the five major organs, a decreased trend was found during this period. Bisulfite sequencing analysis of the CpGs upstream of the promoter showed they were unmethylated. Furthermore, a SNP ( single nucleotide polymorphism)was found between the strains of C57Bl/6J(B6) and Balb/C mice, the direct sequencing of PCR products revealed that this gene was a bioallelic gene in brain, heart and lung. Our results suggest that 2810025M15Rik was a bioallelic gene, it had a wide expression throughout the embryonic development, may play an important role in the mouse embryonic development.
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