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The Comparative Study of Effects of Endurance Exercise, Resistance Exercise and Mixed Exercise on ERK1/2、mTOR、P70S6K mRNA Expression in Skeletal Muscle of C57 Mouse

Author: ZhangJingHong
Tutor: LuJian
School: East China Normal University
Course: Human Movement Science
Keywords: Sarcopenia Resistance exercise Endurance Sports Mixed movement Gene Expression ERK1 / 2 mTOR P70S6K
CLC: R87
Type: Master's thesis
Year: 2010
Downloads: 97
Quote: 0
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With China gradually entered the aging society, the elderly, will appear on the structure and function of the body of many tissues and organs of the recession, which is an obvious sign of skeletal muscle aging. Skeletal muscle is the most important movement in the human body organs, skeletal muscle function decline in the aging process is an important symbol of aging. Aging and the further decline of muscle mass, strength and quality, usually caused by aging due to this change of the muscle called muscle attenuation syndrome (Sarcopenia). Activity due to muscle loss caused the loss indicates that there will be more physical disability and mortality occur, and these in turn with the personal quality of life, social and health care needs of the poor. Sarcopenia-based serious threat, its depth of the molecular biological mechanisms and means of prevention is the focus of current research and hot. Sports is an integral part of the human health lifestyle, in the prevention and slow muscle aging process plays an important role in the movement especially regularity resistance exercise a certain extent, slow or even reverse the aging of muscles, delayed Sarcopenia further development and increase muscle size, strength and non-adipose tissue weight, muscle health promotion of the elderly. Although a variety of sports training on skeletal muscle effects of differences, but also unusual molecular mechanism of different sports on animal or human myogenic response and muscle hypertrophy capacity of cell signaling and muscle hypertrophy pathway changes and the degree of change is influenced by the movement patterns limited knowledge. This article by observing resistance exercise, endurance exercise, endurance exercise combined with anti-group exercise on skeletal muscle C57 2-month-old male mice 28 weeks after the intervention of ERK1 / 2, mTOR, p70S6K mRNA levels expression changes, designed to explore the different nature The degree of change in the effect of exercise on skeletal muscle protein synthesis increased effect and different muscle, slow skeletal muscle aging movement and feasibility for the development of exercise prescription can significantly enhance muscle strength as well as select a valid cause muscle hypertrophy and according to the theory. Experimental Methods: This study selected 32 2-month-old C57 male mice for the study, were randomly divided into the quiet control group (C), endurance exercise group (E), weight ladder group (R), mixed exercise group (ER) n = 8. The selected object, using a treadmill and weight ladder as endurance and resistance exercise mode, for a period of 28 weeks. ERK1 / 2, mTOR, p70S6K mRNA expression level was measured using RT-PCR detection method. Results: 1, motion significantly reduces the weight of the mice. 2, compared to the quiet group, endurance group, resistance groups and mixed groups of mice gastrocnemius of wet weight to body weight ratio (G / W) showed significantly increased in; the quadriceps wet weight to body weight ratio ( V / W) are similar to their situation. 3, ERK1 / 2 mRNA expression levels in the gastrocnemius muscle of the resistance group compared with the other three groups, were highly significantly increased; similar resistance group within the quadriceps, a mixed group expression quieter group and endurance group, decreased significantly. 4, the resistance of mice gastrocnemius mTOR mRNA expression levels, showed a very significant increase compared with the other three groups; mTOR mRNA expression levels of resistance within the quadriceps group is relatively quiet in terms of the group and the mixed group, was extremely significantly increased. The endurance group relative to the mixed group showed a significant increase. Resistance mice gastrocnemius p70S6K mRNA expression levels compared to the other three groups were highly significantly increased. The endurance group relative to the mixed group showed a significant increase. Mixed group expression amount quieter group was significantly decreased; p70S6K mRNA expression levels of resistance within the quadriceps group quieter group showed a significant increase, rather than the endurance group and the mixed group increased very significantly. 6 mice in each group gastrocnemius ERK1 / 2 mRNA expression levels and their corresponding groups quadriceps ERK1 / 2 mRNA expression compared to the amount of expression was significantly higher than the quadriceps expression of resistance group gastrocnemius; mTOR mRNA expression levels in terms of the amount of expression in the resistance group gastrocnemius electrode significantly higher than the expression levels within the quadriceps; the resistance group p70S6KmRNA expression mTOR mRNA expression is similar, but the other group, its expression in the gastrocnemius were significantly higher than the amount of expression within the quadriceps. Conclusion: 1, movement influenced the change in body weight of the mice, the quiet control group, weight gain generally due to the increased fat; minimal changes in body weight and endurance exercise group, generally due to the fat consumed more cause. 2, three different forms of exercise are the mice gastrocnemius and quadriceps ratio increases, the increase in endurance sports group may be due to the relative reduction of fat; resistance exercise group, the increase is due to the quality of the muscle itself due to the increase; increase in mixed sports group may be due to a the fat relative reduction in the muscle itself increase quality. Three sports mode, resistance exercise mice gastrocnemius and quadriceps ERK1 / 2, mTOR, p70S6KmRNA expression were highly significant increase, suggesting that the mode of resistance exercise so far is the effective activation of the signal transduction pathway of protein synthesis promote muscle hypertrophy and delay the the Sarcopenia occurrence of preferred or best sports way. 4, endurance sports mode of mice gastrocnemius or quadriceps ERK1 / 2, mTOR, p70S6K mRNA expression of no significant impact, suggesting that the resistance in skeletal muscle aging, increase muscle mass, endurance exercise in the form of little significance, there is no real desirability. The hybrid sport mode mouse gastrocnemius and quadriceps ERK1 / 2, mTOR, p70S6K mRNA expression of quantity occurred decline Description endurance Games to suppress the effect of resistance exercise, suggesting that increase muscle mass, slow muscle aging trained to properly handle the relationship of both sports mode, and pay attention to the time interval between them and the respective exercise. Gastrocnemius muscle of ERK1 / 2, mTOR, p70S6K mRNA expression level is always greater than the amount of expression in the quadriceps, illustrate the amount of the anabolic genes mainly fast-twitch type II muscle fibers in the expression always higher than its expression in mixed muscle fiber type Ⅰ considerable proportion of type II muscle fibers, biological effects prompted the movement to increase skeletal muscle mass occurs mainly in type II muscle fibers.

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