|
The red deer is the national II level key protection of wild animals , is also an important economic and deer of temperate forest ecosystems play an important ecological role . In recent years , due to the survival of the environment being as more and more serious damage , red deer and the number of sharp decline , research and conservation of existing populations become the focus of future research . The main purpose of this paper is to apply from the northeast red deer droppings pumping proposed quality DNA stool Molecular Ecology and materials , by analyzing microsatellite loci genetic polymorphism of Northeast Red Deer Northeast horse the genetic data of the deer , the Northeast Red Deer individual identification , gender identification , winter home range range , kinship , and between groups of individuals exchange research and to provide a basis for future research and conservation of the species . The experiment in January 2009 to March Wandashan eastern forest region in Heilongjiang Province collected fecal samples of 176 wild Siberian red deer using fecal DNA extraction kit (QIAamp DNA Stool Mini Kit) for DNA extraction , inspection , 176 DNA samples were extracted successfully . 4 pairs of highly polymorphic primers screened from 18 microsatellite primers in closely related species of the deer family , and combined with the preliminary work of the laboratory screening of five pairs of primers for PCR amplification . Genotyping and individual identification obtained 176 fecal samples from 37 different individuals of red deer . SRY primers gender identification , and the results showed that 23 of 37 red deer doe 14 Bucks , sex ratio of 1.00:0.61 . Cervus 3.0 software parental rights the draw 9 group parent - the parent - child relationship , and a group exists inbreeding . The combination of field survey data , the experimental analysis of 8 Red Deer subsets , A and D group of male and female mixed group of female groups B, C and E group , male groups F, G and H group . This experiment 16 the Red Deer individual 's home range , the average female red deer home domain 116.98hm2, average home range of male red deer for 329.08hm2 average male home range area of ??approximately twice that of the female . The group home domain area between 22.62hm2 ~ 383.28hm2 . Frequent exchanges between groups of individuals , the frequent exchanges of separate activities of individuals and groups , and female individuals moved to vacate phenomenon .
|