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The Expression of RECK、MMP-2、TIMP-2 and P21ras in Laryngeal Carcinoma and the Clinical Significance

Author: LiDan
Tutor: HuJunLan;ZhaoRuiLi
School: Hebei Medical University
Course: Otorhinolaryngology
Keywords: Laryngeal RECK Matrix metalloproteinase -2 ( MMP - 2 ) Matrix metalloproteinase inhibitor -2 ( TIMP - 2 ) P21ras Immunohistochemistry Flow cytometry
CLC: R739.6
Type: Master's thesis
Year: 2010
Downloads: 50
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Abstract


Objective: laryngeal cancer is the Department of Otolaryngology - Head and Neck Surgery common malignant tumor. The occurrence of laryngeal cancer and other malignancies, is caused by changes in the level of expression of many genes. Kazal domains associated cysteine-rich reversal inducing protein (reversion-inducing-cystein-rich protein with kazalmotifs, RECK) Gene, is newly discovered in recent years, transcription suppressor gene, the expression of MMPs can inhibit a variety of inhibition tumor invasion and metastasis. Matrix metalloproteinases (matrixmetalloproteinases MMPs) can degrade the extracellular matrix, studies have shown that MMP2 expression level of tumor invasion and is closely related to the degree of malignancy, TIMP-2 as an inhibitor of MMP-2, MMP-inhibition in a variety of ways 2 activity. RECK by the other oncogenes P21ras regulation. In this study, the determination of RECK, MMP-2, TIMP-2 and P21ras in laryngeal cancer paraneoplastic expression in normal tissues and their relationship to explore the occurrence of laryngeal cancer invasion and metastasis. Methods: Using immunohistochemistry (immunohistochemistry, IHC) SP method detected 40 cases of laryngeal carcinoma, 15 cases of adjacent tissue and 15 cases of normal laryngeal mucosa RECK and MMP-2, TIMP-2, p21ras expression. 2 by flow cytometry (with Flow Cytometrye, FCM) method for quantitative detection of 40 cases of laryngeal carcinoma, 15 cases of adjacent tissue and 15 cases of normal laryngeal mucosa RECK and MMP-2, TIMP-2's content. 3 the use of statistical software SPSS13.0 data, for statistical analysis. To P ≤ 0.05 for the difference is statistically significant, P ≤ 0.01 for the difference is statistically significant. : RECK protein expression 1.1 immunohistochemical method: SP immunohistochemical detection of laryngeal carcinoma, cancer tissues and normal mucosa, RECK expression intensity of an upward trend, compared to three groups have significant difference (P lt; 0.01) . Further RECK expression in pairwise comparison, laryngeal carcinoma were lower than the normal group (P lt; 0.01) and the adjacent group (P lt; 0.01), compared to the normal group and the adjacent group had no significant difference (P GT ; 0.05). RECK protein expression in laryngeal carcinoma with lymph node metastasis (P lt; 0.05), clinical stage (P lt; 0.01) and pathological grade (P lt; 0.05), and with the clinical classification, tumor size, smoking, age and sex (P gt; 0.05) 1.2 Flow cytometry: detection RECK protein in laryngeal carcinoma and adjacent tissues and expression in normal mucosa (FI) were 0.8682 ± 0.1961,0.9889 ± 0.1785,1.1200 ± 0.1153, compared to three groups have significant difference (P lt; 0.01). RECK protein expression in laryngeal carcinoma was significantly lower than adjacent tissues (P lt; 0.01) and normal laryngeal mucosa (P lt; 0.01), while the adjacent tissues and normal laryngeal mucosa was no significant difference (P GT ; 0.05). RECK protein expression in laryngeal carcinoma with lymph node metastasis (P lt; 0.05), clinical stage (P lt; 0.01) and pathological grade (P lt; 0.05). MMP-2 protein expression 2.1 immunohistochemical method: the SP immunohistochemical detection of laryngeal carcinoma, cancer tissue and normal mucosa MMP-2 protein expression intensity of a downward trend, compared to three groups have significant difference (P LT ; 0.01). Further pairwise comparisons, laryngeal cancer group, the expression of MMP-2 protein is higher than the normal group (P lt; 0.01) and the adjacent group (P lt; 0.01), while the normal group and the adjacent group there was no significant difference (P gt; 0.05). In laryngeal carcinoma, MMP-2 protein expression and lymph node metastasis (P lt; 0.05), clinical stage (P lt; 0.01) and pathological grade (P lt; 0.01), and clinical type, tumor size, the amount of smoking , regardless of the age and sex (P gt; 0.05) 2.2 Flow cytometry: detection of MMP-2 protein in laryngeal carcinoma and adjacent tissues and expression in normal mucosa (FI) were 2.6088 ± 0.1954,1.0322 ± 0.1577,0.6811 ± 0.1732, compared three groups compared to There are significant differences (P lt; 0.01). MMP-2 protein expression in laryngeal carcinoma was significantly higher than the adjacent tissues (P lt; 0.01) and normal laryngeal mucosa (P lt; 0.01), while the adjacent tissues and normal laryngeal mucosa was no significant difference ( p gt; 0.05) In laryngeal carcinoma, MMP-2 protein expression and lymph node metastasis (P lt; 0.05), clinical stage (P lt; 0.01) and pathological grade (P lt; 0.01) Expression of TIMP-2 protein expression 3.1 immunohistochemical method: application the SP method laryngeal carcinoma, cancer tissue and normal mucosa TIMP-2 expression intensity of a downward trend, compared to three groups have significant difference (P lt; 0.01). Further pairwise comparisons, TIMP-2 expression in laryngeal carcinoma were higher than normal group (P lt; 0.01) and the adjacent group (P lt; 0.01), while no significant difference compared to the normal group and the adjacent group ( p gt; 0.05) In laryngeal carcinoma, TIMP-2 protein expression and lymph node metastasis (P lt; 0.01), clinical stage (P lt; 0.01) and pathological grade (P lt; 0.01), with clinical type, tumor size, smoking regardless of the amount, age and sex (P gt; 0.05) 3.2 Flow cytometry: Detection of TIMP-2 protein in laryngeal carcinoma and adjacent tissues and expression in normal mucosa (FI) were 3.6077 ± 0.1966,1.1125 ± 0.1672,0.6080 ± 0.1987, three groups there was significant difference (P lt; 0.01). The amount of TIMP-2 protein expression in laryngeal carcinoma was significantly higher than the adjacent tissues (P lt; 0.01) and normal laryngeal mucosa (P lt; 0.01), while the adjacent tissues and normal laryngeal mucosa was no significant difference ( p gt; 0.05) TIMP-2 protein expression in laryngeal carcinoma with lymph node metastasis (P lt; 0.01), clinical stage (P lt; 0.01) and pathological grade (P lt; 0.01). The 4 of P21ras protein expression: SP immunohistochemical detection of laryngeal carcinoma, cancer tissues and normal mucosa P21ras expression intensity of a downward trend, a significant difference (P lt; 0.01) compared to three groups. Further pairwise comparisons the laryngeal carcinoma P21ras expression is higher than the normal group (P lt; 0.01) and the adjacent group (P lt; 0.01), compared to the normal group and the adjacent group there was no significant difference (P GT ; 0.05). In laryngeal carcinoma, of P21ras protein expression and lymph node metastasis (P lt; 0.01), clinical stage (P lt; 0.05) and pathological grade (P lt; 0.01), and with the clinical classification, tumor size, smoking, age and sex (P gt; 0.05) 5 RECK and MMP-2, TIMP-2, P21ras in laryngeal carcinoma 5.1 RECK and MMP-2 in correlation: RECK protein expression (FI value) and MMP-2 protein expression (FI) was negative correlation, linear regression equation Y = 2.206X 4.076 (r = 0.895, P = 0.000). 5.2 RECK and TIMP-2 was no correlation (r = -0.208, P = 0.198 gt; 0.05) RECK 5.3 P21ras showed a negative correlation (r = -0.332, P = 0.036 lt; 0.05) Conclusion: the RECK 1 protein, MMP-2 protein, TIMP-2 protein and P21ras protein in laryngeal carcinoma, adjacent tissues and normal laryngeal mucosa were expressed. 2 MMP-2 protein, the qualitative expression of TIMP-2 protein and P21ras protein in laryngeal carcinoma were significantly higher than the adjacent tissues and normal laryngeal mucosa, quantitative and qualitative expression of RECK protein in laryngeal carcinoma were significantly lower than adjacent tissues and normal laryngeal mucosa may be associated with the occurrence of laryngeal cancer. RECK protein expression of MMP-2 protein, TIMP-2 protein and P21ras protein in laryngeal carcinoma with clinical stage, lymph node metastasis, histological grade correlation; clinical classification, tumor size, age, smoking and sex nothing to do. Tip four proteins may be related to the invasion and metastasis of laryngeal cancer. 4 RECK and MMP-2 in laryngeal carcinoma protein expression was negatively correlated with, of RECK and TIMP-2 linear correlation, RECK and P21ras linear negative correlation, suggesting that exists between the four proteins regulate each other and interact.

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