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Experimental Study of Postoperative Radiotherapy on Graft Bone Healing

Author: LiangJia
Tutor: LiuMing
School: Hebei Medical University
Course: Oncology
Keywords: Radiation therapy Bone graft Animal Model Histological Biomechanics
CLC: R687.3
Type: Master's thesis
Year: 2010
Downloads: 14
Quote: 0
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Abstract


Objective: To establish an animal model of the bone graft, the surgical area as the irradiation target simulation postoperative radiotherapy, bone healing observed after irradiation. Trying to analysis after radiotherapy implant bone healing process of information and data, so as to better clinical postoperative radiotherapy, reduce complications and provide a scientific basis. Methods: healthy, mature 36 New Zealand white rabbits, weighing 2 to 2.5kg the rabbit age of 12 to 15 months. In experimental animals, the left anterior superior iliac spine as a starting point, the outward rotation of 30 degrees, a fan-shaped bone cut, approximately 2 cm in radius. Repeat on the right side of the surgery. Cross-transplant bone will be taken to the opposite side of postoperative bone defect fixed suture. To one side to accept irradiation, the other side may be scattered radiation and systemic systematic, on one side only to participate in experimental research. Experimental observation area to the left side of the surgical field, after two weeks in the experimental group the surgical area on the left side of a one-time the 15Gy dose radiotherapy, not the control group. For the experimental observation area to the left side of the surgical area, were randomly divided into two groups: experimental group and control group (n = 18), respectively, disposable surgical area on the left side in the experimental group 2 weeks after radiotherapy 15Gy dose control group will not do the operation. Respectively, after 4 weeks, 8 weeks, 16 weeks, six randomly selected from each group, a batch of 12 to give the X-ray detector, after completion of the X-ray examination of the experimental animals were sacrificed, bone density, biomechanical, gross and histological observation and comparison. Results: 1 X line observation: 4 weeks after surgery, X-ray image of the experimental group showed blurred edges, connected at the point-like callus, rendering low-density, local transmission shadow. X-ray image of the control group showed clear edge, junction callus linear low density around. 8 weeks after surgery, the experimental group 4 experimental animals X first image performance edge unclear connections for linear callus was around the low density, two significant linear callus. The control group of new bone specimens borders slightly fuzzy, rough, a large number of callus formation, five of them showing the connection moth-eaten change. 16 weeks after the X-ray image of the two groups of experimental animals showed sharp edges, the remote bone tissue boundaries, the difference is not obvious. 2 dual-energy X-ray absorptiometry measurements: bone mineral density measurement results of variance analysis showed that the experimental group 4,8,16-week bone mineral density values ??were 0.265 ± 0.08,0.283 ± 0.09,0.295 ± 0.04, control group at each time data 0.287 ± 0.04,0.296 ± 0.06,0.301 ± 0.05. Experimental group and the control group BMD difference, P lt; 0.05, the difference was statistically significant. 3 biomechanical observations: the healing of the bone surface of the specimen shear stress tests, the formula: τ = Q / F. 4 weeks experimental group measured the shear strength of a standard deviation of 0.8462 ± 0.235, and the control group was 1.1083 ± 0.00385, P lt; 0.05, both statistically significant. 8 weeks experimental group was 1.651 ± 0.350, the control group was 1.8403 ± 0.398, P lt; 0.05, the difference was statistically significant. 16 weeks experimental group was 1.9434 ± 0.0158, the control group was 1.9985 ± 0.202, P gt; 0.05 gross and histological observations 4.1 General observation after 4 weeks, the experimental group transplanted bone specimen surface is covered with a fibrous capsule the control group, the specimen surface are covered with a small amount of bone. After 8 weeks, the experimental group 4 bone graft specimens see a small amount of bone covering more than 2 surface is covered with a fibrous capsule, no significant bone; control group transplanted bone specimen surface were more bone coverage. 16 weeks after the experimental group and the control group transplanted bone surface covered with a layer of cortical bone, the differences between the two groups appearance. 4.2 Histological observation of 4 and 8 weeks, the experimental group, the percentage of mature bone cells results was 42.46 ± 8.37,50.25 ± 6.63, control group to 50.35 ± 5.79,52.57 ± 4.47, experimental group and control group mature bone percentage is significantly different, P lt; 0.05, the difference was statistically significant; similar to the experimental group and 8 weeks with the control group at 4 weeks. 16 weeks, the results of the experimental group was 53.65 ± 1.41, the results of the control group, 54.87 ± 1.08, the difference was not significant, P gt; 0.05, the difference was not statistically significant. Conclusion: after conventional dose radiotherapy for bone graft healing as early as the mid different degrees of inhibition, but did not completely prevent bone healing, experimental post two sets of specimens have reached the bone healing. 2 bone graft postoperative radiotherapy is feasible. The case of conventional dose, the bone graft healing after surgery early and mid lagging behind, should pay attention to the clinical situation. 3 radiotherapy as an important means of treatment of bone tumors, the experimental part of the clinical studies to provide a reference.

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CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Orthopedic surgery and surgery > Bone surgery
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