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The problem is that usually appear in pearl cultivation: high mortality, high off the rate of nucleation rate and low priority beads. The existence of these problems resulted in a decline in pearl production benefits. Pearl cultivation process the implanted beads nuclear the pearl oyster vivo pearl oyster injury. Anesthesia method is commonly used to ease surgical stress surgery, planting nuclear process is similar to surgery, anesthesia methods may be inserted in the pearl oyster nuclear operations play a certain role. To improve the penguin pearl oyster the free beads nurture success rate, the purpose of this experiment is to find a means of fast and effective, safe and non-toxic side effects of anesthesia used penguin pearl oyster surgery when anesthesia. Experiments using cryogenic five kinds of narcotic drugs (menthol, ethylene glycol phenyl ether, benzocaine, eugenol, magnesium sulfate) bath anesthesia anesthesia penguin pearl oyster. Drug bath method of anesthesia, the penguin pearl has a narcotic effect: eugenol, ethylene glycol phenyl ether, benzocaine and menthol. Ethylene glycol phenyl ether, benzocaine achieved better anesthetic effect. Menthol anesthesia slower onset Pteria penguin eugenol stimulated to secrete large amounts of mucus. Cryopreservation on the the poor penguin pearl oyster anesthetic effect, no narcotic effect of magnesium sulfate on the penguins pearl. The experimental results are as follows: 1: different concentrations of ethylene glycol phenyl ether and benzocaine because penguins pearl anesthetic effect. Temperature of 25 ℃, ethylene glycol phenyl ether, benzene, benzocaine concentrations were 1.0,2.0, 3.0mlL-1 and 500,800,1000 mgL-1: With the improvement of the concentration of the anesthetic, Pteria penguin anesthesia time required reduced recovery time becomes longer, the 25 ℃, Pteria penguin appropriate anesthetic concentration: benzocaine 800 ~ 1000 mgL-1, ethylene glycol phenyl ether 2.0mlL-1, non-toxic side effects of anesthesia safety. 2: different temperatures anesthetic anesthetic effect. Temperature is set to 21 ° C, 23 ° C, 25 ° C, 27 ° C gradient anesthetic for 1000 mgL-1 benzocaine and 2.0mlL-1 glycol phenyl ether, the results showed that: a certain concentration of the anesthetic, as the temperature rise, reduced anesthesia time, recovery time is reduced. The 3:2 age shellfish in different specifications Penguin pearl oyster narcotic effect. Select two specifications: high shell were 90-100,120-130 mm result: the 2nd instar dB caused by uneven growth in the size difference was no significant effect of anesthesia results. 4: Both anesthetic toxic effects of prolonged anesthesia Penguin pearl. The experimental use 1000 mgL-1 benzocaine due and 3.0mlL-1 ethylene glycol phenyl ether anesthesia time was 15min, 30 min, 45 min, 60 min: benzocaine compared glycol benzene ether penguins pearl poison strong side effects. 5: 500,1000 mgL-1 benzene benzocaine and 2.0mlL-1 glycol phenyl ether anesthesia Pteria penguin at regular intervals along the 5min observation once the anesthetic effect, recording three anesthetic methods were appropriate cut small pieces the length of the time period, the results show: 500 mgL-1 benzocaine better.
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