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The Effects of Visfatin on Vascular Complications in Diabetes Mellitus and Its Mechanism

Author: LiuQiang
Tutor: DengHuaCong
School: Chongqing Medical University
Course: Internal Medicine
Keywords: visfatin diabetes mellitus vascular complications MCP-1 ICAM-1
CLC: R587.1
Type: Master's thesis
Year: 2010
Downloads: 67
Quote: 0
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Abstract


Objective:To investigate the effects of visfatin on expression of MCP-1 and ICAM-1 in vascular endothelial cells under high glucose condition and its mechanism.Methods:Human umbilical vein endothelial cell (HUVEC) were cultured in vitro and divided into 3 groups: control group (0mmol/L glucose); normal glucose group (5.5mmol/L glucose); high glucose group (25mmol/L glucose), and HUVEC were incubated with human recombinant visfatin in various concentrations (0ng/ml, 10ng/ml, 50ng/ml, 100ng/ml) for 24 hours. Another part of HUVEC incubated with 100ng/ml visfatin and glucose in various concentrations (0mmol/L, 5.5mmol/L, 25mmol/L) for 24 hours with SB203580, p38 mitogen-activated protein kinase (p38MAPK) signal pathway inhibitor, pretreatment for 30 minutes. Expression of MCP-1 and ICAM-1 proteins in HUVEC was examined by enzyme-linked immunosorbent assay (ELISA),expression of p38MAPK mRNA in HUVEC was determined by reverse transcription-polymerase chain reaction (RT-PCR) and the proteins of phosphor-p38MAPK in HUVEC was analyzed by Western blot.Results:Expression of p38MAPK mRNA, phosphor-p38MAPK, MCP-1 and ICAM-1 in HUVEC were significantly promoted by visfatin with dose-dependent response in control group and normal glucose group (P<0.01). Under high glucose condition (25mmol/L), visfatin had a synergetic effect on stimulating the expression of p38MAPK mRNA and the proteins of phosphor-p38MAPK in HUVEC and concentrations of MCP-1 and ICAM-1 released from HUVEC (P<0.05). Under these glucose conditions, the effects of visfatin on expression of the proteins of phosphor-p38MAPK and MCP-1 and ICAM-1 were weakened significantly by SB203580, a specific inhibitor of p38MAPK pathway (P<0.01).Conclusion:Visfatin, which has recently been indentified as a novel visceral adipokine, is a vascular pro-inflammatory cytokines. Our data provide the evidence that visfatin can increase the expression of the inflammatory MCP-1 and ICAM-1 via p38MAPK pathways and High glucose can enhance the effects that visfatin induces the expression of MCP-1 and ICAM-1 in HUVEC, which accelerates dysfunction of vascular endothelial cell.

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
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