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Myostatin (MSTN) control animal skeletal muscle growth and development of important cytokines, using PCR-SSCP and sequencing analysis of 180 large yellow croaker samples MSTN gene exon Ⅰ, Ⅱ, Ⅲ and 3'UTR microsatellite sequence single nucleotide polymorphisms. The results showed that exon I 8 mutation point, respectively 28 (C → T), 51 (T → C), 196 (C → T), 264 (G → T), 270 (G → A ), 348 (C → T), 349 (A → T), 352 (G → A). 28 points C → T mutation caused a corresponding amino acid L → F, 349 sites A → T mutation caused by the corresponding amino acids by T → S, the corresponding amino acid 252 sites G → A mutation caused by the E → K, and other bits point nucleotide mutations are synonymous mutations. The presence of AA, BB, BC, AB, BE, BD, and AE seven genotypes, the rate of gene frequencies respectively 0.46,0.17,0.13,0.12,0.07,0.03,0.02. The gene frequencies of A, B, C, D, E, respectively 0.53,0.34,0.06,0.02,0.01. The gene heterozygosity was 0.60, and the polymorphic information content PIC = 0.446. The statistical results showed that an age of large yellow croaker exon Ⅰ regardless of genotype level polymorphism or mutation of the amino acid level was significantly associated with the large yellow croaker weight body length, while the with age 2 large yellow croaker correlation not significantly. The exon II over 10 polymorphic loci are 29,131,206,261,231,243,265,273,297 and 300. 131 point T → A mutation causes the corresponding amino acid L → Q 265 locus T → C mutations cause the corresponding amino acids from the C → R, 261 sites A → G mutation caused by the corresponding amino acids from S → G, 231 sites T → C mutation causing the corresponding amino acids 273 points C → T mutation causes the corresponding amino acid 297 sites A → G mutation caused by the corresponding amino acids I → V R → W by W → S 243 sites G → A mutation causing the corresponding amino acid D → N, 300 sites G → A caused by mutations in the corresponding amino acids by E → K. A → G of the 29 points and 206 points C → A are synonymous mutations. The presence of AA, AB, AC, AD, AE, CC 6 genotypes, genotype frequencies are 0.57,0.23,0.10,0.04,0.02,0.04, allele A, B, C, D, E frequency respectively 0.75,0.12,0.10,0.03,0.01. The heterozygosity of the gene locus was 0.33. Polymorphic information content PIC = 0.41. The statistical results showed that exon Ⅱ, large yellow croaker regardless of the genotype level of polymorphism or mutation of the amino acid level with the weight and length of the large yellow croaker no significant correlation. Exon III has six mutated sites, respectively, 57 (C → T), 104 (A → G) and 122 (T → C), 183 (C → T), 265 (C → G), 348 (T → C). 57 points C → T mutation causes the corresponding amino acids by the Y-→ C the corresponding amino acids 122 locus T → C mutation caused by F → S, 265 sites of C → G mutation causes the corresponding amino acids from P → A, other sites base mutations are synonymous mutations. The presence of exon III 6 genotypes AA, AB, AC, AD, AE genotype frequencies were 0.62,0.20,0.09,0.04,0.04. Allele A, B, C, D, E, frequencies were 0.81,0.10,0.05,0.02,0.02. The gene heterozygosity was 0.33, and the polymorphic information content PIC = 0.31. The statistical results showed that the genotype level and amino acid levels in large yellow croaker exon Ⅲ polymorphism and there is a significant correlation between weight and length of the large yellow croaker. Microsatellite sequences between 64 ~ 126bp in length, the common form of nucleotide substitutions is C → T and A → G, a small number of individual C → G and C → A nucleotide replacement type will appear in the present experimental population detected 25 alleles from different allele frequencies, calculated by the gene heterozygosity 0.941, polymorphic information content of 0.938. The statistical analysis showed that the weight and length of the the microsatellite sequence length and large yellow croaker was positively correlated base substitutions and the weight and length of the large yellow croaker no significant related.
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