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Objective: To study lamininβ1 gene and protein in cellular models of intractable epilepsy expression, α-asarone intervention on refractory epilepsy lamininβ1 neuronal morphology and expression, and to explore α-asarone antiepileptic mechanism of action . Methods: neonatal SD rat, hippocampus rapidly removed after decapitation, serum-free medium nerve (neurobasal medium, NB) and B-27 factor cultured hippocampal neurons; first seven days, the use of neurofilament protein ( neurofilament protein, NF) staining neurons and calculate purity; first nine days, the cultured cells were randomly divided into normal control group (A), model group (B), the model 7.5μg/mlα- asarone group (C group), the model 15μg/mlα- asarone group (D), the model 30μg/mlα- asarone group (E), the model 2μl ethanol group (F), inverted phase contrast microscope to observe the group of neurons morphological changes, automatic biochemical analyzer to detect the cell culture fluid lactate dehydrogenase (lactic acid dehydrogenase, LDH) after modeling 12h, 24h, 48h changes in fluorescence quantitative PCR (FQ-PCR) detection lamininβ1 mRNA expression was detected by immunohistochemistry lamininβ1 protein expression changes. Results: (1) cell models appear intractable epilepsy neuronal migration, integration. (2) α-asarone cell culture medium can be reduced level of activity of LDH. (3) In 12h model group, lamininβ1 mRNA expression occurred (P <0.05), and increased expression with time; at 24h model group, the concentration of α-asarone inhibited lamininβ1 mRNA overexpression (all P <0.05). (4) Lamininβ1 protein mainly in the cytoplasm and extracellular matrix expression, average optical density values: normal control group (0.0947 ± 0.0259), model group (0.1351 ± 0.0262), model 30μg/mlα- asarone group (0.0979 ± 0.0328) , model group and control group was statistically significant difference between (P <0.01), model group and model 30μg/mlα- asarone statistically significant difference between groups (P <0.01). Conclusion: a cell model of refractory epilepsy and the presence lamininβ1 gene overexpression and neurological changes in the grid. 2 cell model of intractable epilepsy neuronal injury exists, with time, the more severe neuronal damage. 3α-asarone inhibited cell model lamininβ1 refractory epilepsy gene and protein overexpression.
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