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The Effect of Unilateral Adrenalectomy on Transfor-mation of Adrenal Medullary Chromaffin Cell in Asthmatic Ratsand the Interference of Kidney-Tonifying Recipe

Author: ZouYeQiang
Tutor: HuChengPing
School: Central South University
Course: Department of Respiratory Medicine
Keywords: Unilateral adrenalectomy Bushen Asthma Adrenal medulla chromaffin cells Neurons
CLC: R562.25
Type: Master's thesis
Year: 2011
Downloads: 26
Quote: 0
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Abstract


Objective: This study was designed to investigate the model through the establishment of deficiency unilateral adrenalectomy on asthma rat adrenal pheochromocytoma and the effect of changes in the intervention of Bushen, thus further revealing chromaffin cell phenotype changes with asthma relationship. Methods: OVA sensitized, inspired method to establish asthma model, 56 male SD rats were randomly divided into seven groups, namely asthma, unilateral adrenalectomy asthma group (surgery asthma), kidney asthma group (Bushen group) unilateral adrenalectomy kidney asthma group (kidney surgery group), dexamethasone asthma group (ground rice group), unilateral adrenalectomy dexamethasone asthma group (surgery to m group) and normal control group is E. Whole body plethysmography using rats in each group was detected by changes in airway resistance; rats were observed by light microscopy pathological changes in the adrenal medulla, electron microscopy the rats adrenal medulla chromaffin cell ultrastructure; immunohistochemistry assay of the adrenal medulla phenylethanolamine-N-methyltransferase (Phenylethanolamine N-methyl transferase, PNMT), GDP dissociation inhibitor (Rho GDP dissociation inhibitorα, Rho GDIα) and peripheral proteins (Peripherin) expression; RT -PCR and western blot were used to detect adrenal medulla Rho GDIα, Peripherin mRNA and protein expression levels; ELISA assay of serum epinephrine (Epinephrine, EPI) level changes. Results: 1. Compared with normal control group, asthma group rat adrenal medulla obvious structural damage, degeneration or lipid vacuoles increased airway inflammatory cell infiltration seen around; surgery asthmatic rats and adrenal medulla structural damage to the lungs further exacerbated asthma group compared, kidney group and ground rice group adrenal medulla and lung structural damage compared to alleviate asthma; asthma compared with surgery, kidney surgery group and surgery group to m structural damage to the adrenal medulla and lungs lighter. Electron microscope, the control group, the adrenal medulla chromaffin cell morphology structured, mitochondrial structure is clear, the number of chromaffin granules and more uniform density; compared with the control group, asthma group pheochromocytoma cytosolic and mitochondrial swelling, secretory granules decreased; surgery In addition to the asthma group performance, but also shows medullary chromaffin cells and apoptotic processes like change, and kidney group and the extent of the damage to rice group structure has been reduced compared with the asthma group; asthma compared with surgery, kidney surgery and surgical group ground rice group adrenal medulla chromaffin cell lesions reduced. (2) Compared with control group, asthmatic rats medulla chromaffin cells PNMT EPI expression and serum levels were significantly lower (P lt; 0.05); asthma group, asthma group than in the surgery to further reduce the asthma group ( P lt; 0.05), while the kidney group and ground rice group PNMT and EPI increased expression (P lt; 0.05); asthma compared with surgery, kidney surgery group and its operation to m group increased expression (P lt; 0.05). 3.Rho GDIα trend with PNMT expression is similar in the control group, Rho GDIα a clear expression; compared with the control group, asthma group was decreased (P lt; 0.05); compared with asthma, asthma surgery further reduced compared with the asthma group (P lt; 0.05), while the kidney group and ground rice group expression increased (P lt; 0.05); asthma compared with surgery, kidney surgery and surgical group increased expression of ground rice group (P lt; 0.05 ). 4 in each group rat adrenal medulla chromaffin cells are visible Peripherin expression, compared with controls, Peripherin increased expression in asthma group (P lt; 0.05); asthma group compared with the surgery group, the expression of asthma further increased (P lt; 0.05), kidney group and the group was reduced to m (P lt; 0.05); asthma compared with surgery, kidney surgery group and surgery group was reduced to m (P lt; 0.05). Conclusions: 1. Unilateral adrenalectomy can exacerbate asthma rats AMCC structure and function of the change, but also can promote the transformation into neurons AMCC. 2 Bushen can inhibit asthmatic rat adrenal medulla chromaffin cells into neurons change process. 3Rho GDIα and Peripherin expression changes with changes in adrenal medulla chromaffin cells of the question, there is a close relationship.

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CLC: > Medicine, health > Internal Medicine > Respiratory system and chest diseases > Trachea and bronchial disease > Bronchial disease > Bronchial asthma
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