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Objective: To study ephedra water extract nebulized bronchial asthma airway inflammation and leukocyte interleukin -13 (IL-13), eosinophil chemotactic factor (Eotaxin) in asthmatic mice airway expressed. Explore the mechanism of action of ephedra treatment of asthma, to provide the experimental data and the theoretical basis for ephedra atomization inhalation treatment of asthma. Methods: (1) ephedra plus water immersion 30min Jianzhi 1h concentrated, dubbed the concentration equivalent to the decoction of the crude drug 1g/ml water, and stored at 4 ° C refrigerator spare. (2) select 24 healthy female BALB / c mice (5-6 weeks old), weighing 16 ~ 20g, were randomly divided into three groups: control group (A), asthma group (B), ephedra treatment group ( Group C), 8 / group. Except group A, B, C groups were established bronchial asthma animal model. 0,7 d intraperitoneal injection of ovalbumin antigen solution of 0.2 ml (containing Al (OH) 3lmg and ovalbumin 100μg) sensitization 14d daily ovalbumin intranasal inhalation 40μl2% saline solution will be excited, 1 / d, Continuous 7d. C mice in each intranasally before stimulation 1h I ephedra decoction 50ml atomization inhalation 30min, 2 times / d, continuous 9d. A group with normal saline instead of antigen solution was injected intraperitoneally, inspire and inhalation therapy in group B with normal saline inhalation therapy. Respectively in modeling the 21d and 23d abdominal anesthesia lavage specimens from lung tissue samples. (3) counts in bronchoalveolar lavage fluid (BALF) of the total number of leukocytes and EOS total number; lung tissue HE staining, optical microscope to observe the pathological changes of the bronchus, bronchial and lung tissue around; immunohistochemical staining (SP) determination of the the bronchopulmonary tissue IL-13, Eotaxin protein; double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection of lung tissues, IL-13, of Eotaxin the protein concentration. Experimental data using the mean ± standard deviation (x ± s) one-way ANOVA and linear correlation analysis using SPSS17.0 statistical software. Results: (1) group B BALF WBC, EOS significantly higher number (P lt; 0.05); compared with group B group C in the WBC, the EOS number of significantly reduced (P lt; 0.05), but still higher than in group A ( P lt; 0.05). (2) conventional HE staining: A group of airway epithelial integrity, bronchi, bronchioles wall smooth muscle thin lumen rules, no secretions storage, the mucosal folds dwarf flat alveolar septa normal around no obvious inflammatory cell infiltration; the B Group airway epithelial cell hypertrophy, epithelial shedding incomplete, smooth muscle thickening, bronchial lumen narrow lumen visible mucus plug, alveolar septal thickening, peribronchial inflammatory cell infiltration; with Group B, C mice bronchial wall thickening and stenosis are to reduce peribronchial and alveolar inflammatory cell infiltration was reduced significantly thinner alveolar septa, alveolar space larger. (3) immunohistochemistry staining: Compared with group A, group B bronchial epithelial cells and stromal cells positive expression cells, deep coloring, and the difference was statistically significant (P lt; 0.05); C The positive expression cells less than in group B, were lighter, and the difference was statistically significant (P lt; 0.05). (4) ELISA results show that: the B group bronchial lung tissue IL-13, Eotaxin concentration was significantly higher than group A and group C, the difference was statistically significant (P lt; 0.05); compared with group B, C mice IL- 13, Eotaxin protein concentration was significantly lower, the difference was statistically significant (P lt; 0.05). (5) the relationship: IL-13 protein expression in BALF EOS count: group B were significantly increased (r = 0.746, P lt; 0.05), C group decreased significantly (r = 0.729, P lt; 0.05) . Eotaxin protein expression in BALF EOS count: group B were significantly increased (r = 0.712, P lt; 0.05), C group were significantly lower (r = 0.734, P lt; 0.05). IL-13 protein expression Eotaxin protein expression: the B group IL-13 and Eotaxin protein concentration were significantly increased (r = 0.803, P lt; 0.05), of C group IL-13 and Eotaxin protein concentration were significantly reduce (r = 0.761, P lt; 0.05). Conclusion: (1) in the presence of IL-13, Eotaxin protein upregulation in the bronchus of murine lung tissue. (2) ephedra aqueous extract aerosol inhalation inhibits airway inflammation in asthmatic mice. (3) Ephedra inhibit the airways of asthmatic mice IL-13 protein expression of Eotaxin may be one of its anti-inflammatory mechanism.
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