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Associaton Study between TNXB Gene Polymorphisms and Schizophrenia in a Chinese Han Population

Author: WangZuoYan
Tutor: ZhangLi；ZhangZuo
School: Jilin University
Course: Neurology
Keywords: schizophrenia/genetics polymorphism single nucleotide TNXB rs204887
CLC: R749.3
Type: Master's thesis
Year: 2011
Downloads: 55
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Abstract

Schizophrenia is a kind of to seriously effect mankind health of familiar disease and more be taken bad. But the outbreak machining of schizophrenia, to the cause of disease plain and concretely pathologic physiology machining is still not clear. Being mankind is complicated of many gene hereditary diseases, the outbreak of schizophrenia is decided by to inherit character and environment factor, the heredity factor has an importance action in deciding easily suffering from sex variation and outbreak, while the action of environment factor is smaller. Therefore, relevant schizophrenia easy feeling gene and it the search of pathologic physiology machining, have been the point and crux of the genetics search realm of schizophrenia.Objective:This experiment makes use of molecule genetics technique and big sample case-check against, thorough study schizophrenia split symptoms choose the relation between many states and schizophrenia of the gene TNXB gene, to further analysis the crowd of northern China Chinese be taken bad with schizophrenia amid TNXB gene many states and it the relation of clinical table type.Methods:All research objects are all northern China Chinese persons.779 sufferers of schizophrenia all are mental provincial governor spring City, Jilin hospitals, cure according to hospitalization of international disease group standard version 10(ICD-10) diagnosis standard by two experienced and clinical doctors of sufferer.983 healthy collates come from health to dedicate a blood, have healthy request and have no serious body disease and the spirit disease household history.At advertise for the parties know what happened the condition of assent down, sample the sufferer of schizophrenia is 5 milliliters, make use of phenol/chloroform with cycle vein blood out of the healthy volunteer lay on to lift the law extract gene set DNA to combine to create a sample library. The adoption comes together the Mao chain type reaction-the method of the restriction segment length many states(PCR-RFLP), examining TNXB gene the is rs204887 dot genotypes.The assessment of the clinical data according to a spirit section assessment quantity table, according to search need to design a series representative disease symptom of variable, create a clinical table type database. The Hardy-Weinberg equilibrium for genotypic distribution was estimated by the goodness-of-fit X2 test. The frequencies of alleles and genotypes of TNXB gene and the relationship between allelic frequencies and the clinical phenotypes of schizophrenia were statistically computed.Results:(1) The goodness-of-fit X2 test showed that the genotypic distributions of rs204887 did not deviate from Hardy-Weinberg equilibrium in both patient group and control group (P>0.05)(2) The equipotential gene correlation analysis expresses that C and T quipotential are in the case set and the matched control repeatedly count rayleing distribution rayleigh compare the discrepancy didn’t show allelic (P>0.05); Sample according to male, female respectively the progress statistics, rs204887 equipotential genes of dots repeatedly count rayleing distribution rayleigh at rayleing distribution rayleigh within the case set and the matched control compare the discrepancy all didn’t show allelic (P>0.05).(3) The genotype correlation analysis expresses that C/C and C/T and T/T 3 kinds of genotypes are in the case set and the matched control of repeatedly count rayleing distribution rayleigh compare the discrepancy did not show allelic (P>0.05); Sample according to male, female respectively the progress statistics, rs 204887 dot genotypes repeatedly count rayleing distribution rayleigh at rayleing distribution rayleigh within the case set and the matched control compare the discrepancy all didn’t show allelic.(P>0.05)(4) In the case set of 779 schizophrenia, rs204887 C and T equipotential and C/C, C/T and T/T the genotype repeatedly count various feminine gender symptom of rayleing distribution rayleigh difference and the sufferer clinical table type of schizophrenia progress the R X C table x 2 inspect, there were no correlations between rs204887 dot equipotential genes and genotype is as dull as thinking lean, emotion as a result/cool with will lack a clinical table type of etc. also irrelevant allied (P>0.05).(5) In the case set of 779 schizophrenia, The C and T equipotential and C/C, C/T and T/T of rs204887 dotses genotype repeatedly counts various male symptom of rayleing distribution rayleigh difference and the sufferer clinical table type of schizophrenia progress the R×C table x 2 inspect, there were no correlations between allelic or genotypic frequencies and the clinical phenotypes of schizophrenia, such as, the true listens to an illusion, relation crazy idea, effect crazy idea, self-reproach from offense crazy idea, unreal crazy idea, read a core disease, kill crazy idea, envy crazy idea, overstated crazy idea, fall in love the articulate logic obstruction, weird behavior and kick of the obstruction, thinking of the crazy idea, thinking to harm person’s behavior (P>0.05)ConclusionThe studies a result prompt:TNXB gene rs204887 dot possibilities have nothing to do with northern China Chinese crowd schizophrenia’s being taken bad to unite, TNXB locus may not be associated with schizophrenia in a Han descent population in the north of China. Although this search can not verify that TNXB gene and schizophrenia is taken bad correlation to unite, combine incapability’s enough and now negating TNXB gene is the probability of easy schizophrenia feeling gene.The further search should select several TNXB gene label SNPs are to inherit a lable progress search and get more accurate conclusion.

Associaton Study between TNXB Gene Polymorphisms and Schizophrenia in a Chinese Han Population

Abstract

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