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Objective: To observe the different doses of puerarin female Kunming mice reproductive system, sexual maturity and the development of the mouse embryo - fetal explore the puerarin reproductive period mice mode of action and its reproductive toxicity, as Pueraria factors in clinical applications to provide experimental evidence. Research methods: (1) mice were observed through the vaginal smear estrous cycle, changes in uterine weight gain was observed in mice uterus, enzyme-linked immunosorbent assay serum estradiol (estradiol given E2) and follicle-stimulating hormone (follicle-stimulating hormone, FSH), luteinizing hormone (luteinizing hormone, LH) and other changes in estrogen levels to assess the impact of puerarin on sexually mature female of Kunming mice reproductive system. 8.9-week-old female Kunming mice 40, according to the random number table, were randomly divided into four groups: low-dose puerarin group (150mg/kg), high dose puerarin group (450mg/kg), diethylstilbestrol group ( 35mg/kg), solvent control group (sodium carboxymethyl cellulose), in each group were administered orally, once a day, every time 0.2ml. 08:00 am daily vaginal smears check, observe and record the changes of the estrous cycle in mice. Weekly weighing the body weight of mice, the mice in each group drug dosage adjusted according to body weight. Administered continuously for 6 weeks, 24 hours after ending administration before estrous, orbital blood, the mice were sacrificed by cervical, fast to said take the wet weight of the uterus, ovaries, uterus coefficient were calculated, ovarian coefficient. All serum samples were stored at -20 ℃, the side of the ovary, uterus quickly move stored at -80 ℃, the other side of the ovary, uterus placed in a solution of 10% formalin fixed paraffin slice HE staining uterine histopathological changes. (2) on pregnant female mice from embryonic implantation to the hard palate closure (mouse pregnancy 6-15 days) treated with different doses of puerarin pregnant female mice, embryonic and fetal assessment puerarin. The experiment 160 Kunming mice 8-9 weeks of age, male and female, according to the random number table female mice were randomly divided into four groups, each group of 20. The same kind of the same month-old male and female mice in the same cage at a 1:1 ratio, to start the next day in the same cage, observe whether the female vaginal opening vaginal plug at 08:00 am every day, for no obvious vaginal plug small mouse vaginal smear should be done to check the presence or absence of live sperm in the female vagina. If it is found that the vaginal plug or vaginal smear sperm positive, given as gestational 0d cages of two weeks, administered orally on days 6-15 of pregnancy. 18 days of pregnancy, cervical dislocation killed pregnant rats, fetal mice separated from the uterus to the top, whether the visual inspection stillbirth absorbed fetus, statistics of the number of live births each nest, check live births with or without the appearance of deformity, bone deformities, visceral malformations calculate the average weight of each nest live births, and average crown to rump length of fetal rat measured with a vernier caliper. Results: (1) Weight: low-dose puerarin group weight was significantly lower than the control group (p lt; 0.05), significantly lower than the high dose the puerarin group Diethylstilbestrol group (p lt; 0.01), high-dose puerarin group, no statistically significant difference between the the diethylstilbestrol group and the control group. (2) organ coefficient: ovarian coefficient between groups was not statistically different; uterine coefficient, diethylstilbestrol uterus coefficient significantly higher than the low-dose puerarin group and the control group (p lt; 0.01), significantly higher than the high dose of Pueraria vegetarian group (p lt; 0.05), low dose puerarin uterus coefficient is lower than that of the control group, but no significant difference in the high-dose puerarin group was higher. (3) mouse estrous cycle: 42 days gavage puerarin group, low-dose, the puerarin group of high-dose, diethylstilbestrol group, the control group of mice estrous cycle disorder rates were 70%, 80%, 100% , 0, showing that low-dose puerarin, high-dose puerarin, diethylstilbestrol could cause the mouse estrous cycle disorders, low dose puerarin group and high dose group of puerarin the estrous cycle disorders was significantly lower than the diethylstilbestrol group. (4) the level of serum E2: low-dose puerarin group was significantly lower than the control group (p lt; 0.05), puerarin group was significantly lower than the high-dose, and diethylstilbestrol group (p lt; 0.01), high-dose puerarin group and diethylstilbestrol group significantly higher than the control group (p lt; 0.01), high doses of puerarin group was significantly lower than the the Diethylstilbestrol group (p lt; 0.01). (5) the level of serum FSH: low-dose puerarin group was significantly lower than the the puerarin group of high-dose, the diethylstilbestrol group and control group (p lt; 0.01), high-dose puerarin group and diethylstilbestrol group was significantly higher than that of the control group and the low dose Pueraria was no significant difference between the hormone group (p lt; 0.01), high dose puerarin group and diethylstilbestrol group. (6) serum LH levels: low-dose puerarin group was significantly lower than that of the high-dose puerarin group, the Diethylstilbestrol group (p lt; 0.01), significantly lower than the control group (p lt; 0.05), high-dose puerarin group and diethylstilbestrol group puerarin group was significantly higher than that of the control group and the low dose (p lt; 0.01), no statistically significant difference between the puerarin group and diethylstilbestrol group of high-dose. (7) The uterine histopathological examination: diethylstilbestrol group uterine hypertrophy, interstitial edema of the endometrial tissue, glands increase in the number of bending enlarged glandular different morphological cell proliferation of the glandular epithelium was tall columnar pseudostratified basal layer slightly thickened, low-dose puerarin group, high dose puerarin group and the control group were not similar histological features. (8) endometrial thickness: diethylstilbestrol group endometrial thickness was significantly higher than the low dose Pueraria group, high dose puerarin group and the control group (p lt; 0.01), low-dose puerarin group, high-dose puerarin group and the control was no significant difference between the groups. (9) pregnant rats weight: 6, 9, 12, 15 days of pregnancy weight was no significant difference between the groups; 18 days of pregnancy weight, diethylstilbestrol group was significantly lower than the control group (p lt; 0.01), significantly lower low dose puerarin group and high dose puerarin group (p lt; 0.05), the puerarin group of low-dose and high-dose puerarin group and the control group was not statistically significant. (10) mouse embryo formation: the number of live births, low dose puerarin group, high dose puerarin group, the number of live births, lower than the normal group, but no significant difference was significantly higher than the the Diethylstilbestrol group (p lt; 0.05) high, no statistically significant difference between the low-dose puerarin group. The the diethylstilbestrol group was significantly lower than the control group (p lt; 0.01); absorbed fetus was no significant difference between the groups; each group were no stillbirths. (11) mouse fetal development: average fetal weight, low-dose puerarin group lower than the high dose puerarin group and the control group, but no significant difference diethylstilbestrol puerarin group was higher than that of high-dose group, lower than the normal group , but no significant difference between the diethylstilbestrol group was significantly lower than the control group (p lt; 0.01); The average fetal crown to rump length, low-dose puerarin group than in the high-dose puerarin group and the control group, higher than the diethylstilbestrol group but no statistics learning differences, high-dose puerarin group than diethylstilbestrol group, lower than the normal group, but no significant difference The diethylstilbestrol was significantly lower than the control group (p lt; 0.01); research conclusions: (1) under the experimental conditions, puerarin on the reproductive system of sexual maturity in mice have the dual role of a certain effect, of puerarin due dose different play estrogen and anti-estrogen. (2) during pregnancy females I puerarin gavage of dams and offspring mice had no significant adverse effects, namely puerarin there is no obvious embryos fetal developmental toxicity.
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