Dissertation > Excellent graduate degree dissertation topics show

Effects of All-trans Retinoic Acid on High Glucose-induced Expression of ACE/ACE2 in Human Renal Tubular Epithelial Cells

Author: MengXue
Tutor: GuoHanCheng
School: Fujian Medical
Course: Internal Medicine
Keywords: all-trans retinoic acid renal tubular epithelial cells angiotensin converting enzyme diabetic nephropathy
CLC: R692.9
Type: Master's thesis
Year: 2011
Downloads: 21
Quote: 0
Read: Download Dissertation

Abstract


Objective To investigate the influence of all-trans retinoic acid（ATRA）on the expression of angiotensin converting enzymes（ACE）,angiotensin converting enzymes2（ACE2）and AngiotensinⅡ（AngⅡ）in human renal tubular epithelial cells induced by high glucose and to probe the role of RAS in the pathogenesis of diabetic nephropathy（DN） and the intervention situation of ATRA.Methods HK-2 cells cultured in vitro were randomly divided into several groups:（1）normal glucose group（NG）, high glucose group（HG） and mannitol group（MG,）.Each group were cultured 24h,48h and 72h respectively;（2）HK-2 cells in HG group were exposed to medium containing 30mmol/L D-glucose and different concentrations of ATRA (ATRA1 group 10-7mol/L、ATRA2 group 10-6mol/L、ATRA3 group 10-5mol/L), or 10-2mol/L ethanol（ethanol group）,or 10-5mol/L captopril（Captopril group）.Each group were cultured for 48h. The expression of ACE and ACE2 mRNA in HK-2 cells were detected by reverse transcription polymerase chain reaction （RT-PCR） and the expression of ACE and ACE2 protein were detected by Western blot . The concentration of AngⅡin the culture medium was detected by enzyme linked immunosorbent assay（ELISA）. Results （1）In comparison with NG group,the mRNA and protein expression of ACE in HG group increased significantly in a time dependent manner （P<0.01）. The expression of ACE2 in mRNA and protein in HG group at 48 hours and 72 hours after induced by high glucose decreased significantly in comparison with NG group （P<0.01）. The level of AngⅡin HG group were higher significantly than in NG group（P<0.05）.（2）The expression of ACE mRNA and protein in HK-2 cells cultured under high glucose joining 10-7mol/LATRA were inhibited significantly in comparison with HG group （P <0.05）, and the inhibition increased with the dose increasing,and this difference was statistical significance （P <0.01）.While 10-7mol/LATRA began reversing the expression of ACE2 mRNA and protein in HK-2 cells induced by high glucose at 48h after joining ATRA （P <0.05）, and the reversal enhanced with the dose increasing. The difference were statistical significance （P <0.01）. The concentration of AngⅡin groups with adding 10-7mol/LATRA already decreased in compared with HG group （P <0.05）,and the concentration of AngⅡin 10-6mol/LATRA group and 10-5mol/LATRA group decreased significantly in compared with NG group （P <0.01）.Conclusion （1）High glucose can induce ACE expression and inhibit ACE2 expression ,then promote synthesis of AngⅡin proximal tubular cells（.2）ATRA can increase the expression of ACE2 and inhibit the expression of ACE, and then inhibit the expression of AngⅡinduced by high glucose in HK-2 cells,which may play an important role in delaying diabetic nephropathy.

Related Dissertations

  1. Low Dose of Spironolactone Reduces Oxidative Stress and Prevents the Development of Diabetic Nephropathy in Patients with Type 2 Diabetes Mellitus,R587.2
  2. Study on the in Vitro Effect of Zinc on the Growth of Murine Embryonic Palatal,R782.2
  3. Human adipose -derived mesenchymal stem cells to treat diabetic nephropathy rat studies,R692
  4. Retinoic acid and matrine intervention rat hepatoma change process β-catenin, E-cadherin, MMP-7 dynamic changes,R735.7
  5. The Changes of T Lymphocyte Subsets and Costimulatory Molecules Before and After Shuxuetong Treatment in Type 2 Diabetes Subjects with Diabetic Kidney Disease,R692
  6. The Role of SIK1 in Type 2 Diabetic Nephropathy with Hypertension,R544.1
  7. The Expression of Angiopoietin-2 in Diabetic Nephropathy and Correlated with Proteinuria,R587.2
  8. Pentamethylquercetin on diabetic nephropathy in rats and its mechanism,R285.5
  9. Relationship between Polymorphism of NAT1、NAT2 and ACE Gene and Hair Dye Dermatitis,R758.2
  10. Analysis of the Relationship between the Expression of SARA and the Pathological Types and Clinical Phenotypes of Diabetic Nephropathy,R587.2
  11. The Curative Effect Comparison between Hemodialysis and Continuous Ambulatory Peritoneal Dialysis on End Stage Diabetic Nephropathy,R587.2
  12. RAS dysfunction induced by high glucose in renal tubular epithelial cells and its mechanism of action,R692.9
  13. Effect of Blood Glucose Fluctuation on Renal Pathological Change and Collagen Ⅳ Expression of Diabetic Rats,R587.1
  14. Effects of Pioglitazone on Serum Osteopontin and Urinary Microalbuminuria in the Type 2 Diabetic Patients with Diabetic Nephropathy,R587.2
  15. N-acteyl-β-D-glucosaminidase Assay Kit and Its Clinical Application,R587.1
  16. Serum 25-hydroxy Vitamin D Level in Diabetic Patients and Its Effect on Blood Glucose and Bone Mass,R587.1
  17. The Experimental Research of the Interference Effect of Schisandra Chinensis Mixture on Glomerular Fibrosis Progress of Diabetic Nephropathy Mice,R285
  18. Protective Effect of Mangiferin on Kidney in Diabetic Rats,R285.5
  19. Professor Wang Mei treatment of diabetic nephropathy spleen Experience,R249
  20. Clinical Observation of Early Stage Diabetic Nephropathy in Treatment with Tonifying Kidney-Thirst Mixture,R259
  21. Study on the Effects of the Mothod of Invigorating the Spleen and Kidney Detoxification and Vigoration of Blood Circulation on Early Diabetic Nephropathy and Flt-1 Expression on Rat Models,R259

CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Kidney disease > Other diseases
© 2012 www.DissertationTopic.Net  Mobile