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Purpose: To investigate the impact of femur fracture with brain injury in rats immune response transplantation of bone marrow mesenchymal stem cells (inflammatory mediators, immune status) and has a protective effect on vital organs. Experimental methods 55 Sprague-Dawley male rats, weighing 250-300g, were randomly divided into a control group, injury group and stem cell transplant group. The control group of 5, 35 CMW group, stem cell transplantation group 35. Injury group and stem cell transplantation group resulting in the right femoral shaft fracture with moderate closed head injury, stem cell transplantation in rats caused Combined Injury 5 hours, from the tail vein injection of bone marrow mesenchymal stem cells 4x106cell /. In 1, 2, 3, 5, 7 days after modeling from the injury group and stem cell transplantation group 5 each rat abdominal aortic blood, plasma IL6 coding for IL-8, IL-10, the detection of circulating blood mononuclear cells surface expression of MHC Ⅱ whole blood after lipopolysaccharide-stimulated synthesis of TNFα, serum aspartate amino transfer enzymes (AST), alanine aminotransferase enzyme (ALT) and creatinine (Cr), blood urea nitrogen (BUN) lactate dehydrogenase (LDH), creatine kinase (CK) content, and the detection of the specific gravity of each rat lung wet-to-dry (W / D). The control group received no treatment, only the detection. Take the control group of five rats, injury group and stem cell transplantation group 1 day 5 after modeling rat lung, liver, heart, kidney, to do a biopsy and HE staining. Changes in immune indicators by comparing changes (inflammatory mediators, monocyte cell surface MHC II expression, whole blood after lipopolysaccharide-stimulated synthesis of TNFα capacity) and vital organs of the organization and function (biopsy, biochemical markers, lung W / D) analysis of bone marrow mesenchymal stem cells for serious injury in rats with the regulation of the immune response and protection of vital organs. Experimental results: 1. Femur fracture with brain injury injury of IL-6, IL-8 increased significantly, especially in the early injury, plasma IL-10 early decline, rebounded to late. Mononuclear cell surface MHC Ⅱ and whole blood in vitro TNFα synthetic capacity in the early injury to a rapid decline in the last day to minimize the period of observation. AST, ALT, BUN, CK peak one day in the trauma group, then gradually decreased, Cr began to increase in the first day of the trauma, the group reached the peak in three days, then gradually decreased, LDH reached in the first three days of post-traumatic peak, then gradually decreased. Trauma rats in early injury, lung wet-to-dry ratio (W / D) was increased late in the observation period, W / D has improved slightly, but still higher than normal. 24 hours after the trauma of the lung, heart, liver and kidney slices visible trauma early vital organs acute injury, found reasonable slice ratings of lung disease, rats femur fracture with brain damage, lung injury in rats integral significantly increased. BMSCs transplantation rats early after injury, plasma IL-6, IL-8 was significantly reduced IL-10 experienced a brief decline, a rebound in the latter part of the observation period, plasma IL-10 or at the level of the control group. BMSCs transplanted rats, immune function (whole blood in vitro synthesis the TNFα capacity, mononuclear cell surface MHC Ⅱ) early injury down quickly, but higher than that the invasive rats of the same point in time. In the latter part of the observation, it can be observed that the upward trend of these two indicators. Serum biochemical markers, lung W / D with respect to the same point in time trauma rats decreased, pathological response of tissue damage relative to the trauma group also alleviate. Conclusion: immunosuppression after bone marrow mesenchymal stem cells can reduce the inflammatory response of severe trauma, and effectively reverse the trauma state, so as to protect the vital organs.
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