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Objective: Renal ischemia-reperfusion injury (renal ischemia / reperfusion injury, RI / RI) is a common clinical pathophysiological phenomenon plays an important role in the pathological process of ischemic acute renal injury, multiple factors involved in the complex pathology process. After ischemic preconditioning (ischemic preconditioning, IPC) refers suffered repeated transient ischemic tissues and organs can be protected in the follow-up of long-term ischemic mitigate ischemic injury. The study found that many organs such as the heart, liver and small intestine, IPC has a protective effect, but its mechanism is not yet fully elucidated. The brief repeatedly IPC whether RI / RI has a protective effect, is rarely reported. Therefore, to investigate the protective effect and mechanism of short repeated IPC RI / RI, has a very important significance for the prevention and treatment of the RI / RI. This study was designed to observe the role of the the brief repeatedly IPC RI / RI application of physiological, biochemical, radioimmunoassay and immunohistochemistry of, and to explore the possible mechanism of action. Methods: 40 healthy adult male SD rats, weighing 150-180g, in 18-22 ° laboratories quiet feeding two days later the animals were randomly divided into five groups: sham group (Sham) 2 ischemic reperfusion group 3 five minutes (I / R), ischemic preconditioning ischemia reperfusion (I / R) 4 five minutes ischemic preconditioning secondary to ischemia-reperfusion (I / R) 5 five minutes ischemic preconditioning three ischemia-reperfusion (3 I / R) animals in each group by 1% sodium pentobarbital intraperitoneal anesthesia, laparotomy, exposure bilateral renal artery and vein. Sham group, only open occlusion of bilateral renal artery and vein; I / R group, clamping bilateral renal artery and vein 45 min pine folder reperfusion; 1 I / R, 2 I / R and 3 I / R in each group were clamping bilateral renal artery and vein five minutes reperfusion five minutes, twice and three times (IPC) after the line clamping bilateral renal artery and vein 45 min after unclamping reperfusion, layer by layer suture the peritoneum, the abdominal muscles and skin. 24 hours after blood, and both kidneys serum creatinine (Scr), blood urea nitrogen (BUN), kidney tissue malonyl aldehyde (MDA), nitric oxide (NO), nitric oxide synthase (iNOS), superoxide dismutase (SOD), endothelin (ET), and the expression of HO-1 in renal tissue. Results: 1 rat RI / RI, light microscopy findings: Sham group kidney tissue was normal; increased I / R renal tissue injury, showed tubular epithelial cells cloudy swelling, necrosis, luminal expansion between qualitative change wide, edema, infiltration of inflammatory cells. 1 I / R group, 2 I / R and I / R groups the degree of renal tubular injury significantly reduced compared with I / R group, interstitial inflammatory cells significantly reduced edema significantly reduced. The degree mitigate damage to 3 I / R group the most significant. The 2 rats RI / RI after I / R renal function decreased BUN and Scr were significantly higher than the Sham (P lt; 0.01); compared with the I / R group, 1 I / R, 2 I / R 3 I / R groups BUN and Scr were significantly lower (P lt; 0.05 ~ 0.01), which reduce the extent to 3 I / R group the most significant. 3 rats RI / RI, the I / R group MDA content than Sham group were significantly increased; SOD activity were significantly lower than Sham group (P lt; 0.01); compared with the I / R group, 1 I / R, 2 I / R and 3 I / R groups SOD activity was significantly increased, and MDA content was significantly lower (P lt; 0.05 to 0.01), which is to reduce the content of MDA to 3 I / R group the most significant. 4 rats RI / RI after I / R group NO and iNOS content was significantly higher than the Sham group (P lt; 0.01); compared with the I / R group, 1 I / R, 2 I / R and 3 I / the R groups content of NO were significantly increased (P lt; 0.05 ~ 0.01), and iNOS content was significantly lower (P lt; 0.05 to 0.01). The 5 rats RI / RI after I / R group ET content was significantly higher than the Sham group (P lt; 0.01); compared with the I / R group, 1 I / R, 2 I / R and 3 I / R groups the ET content significantly decreased (P lt; 0.05 ~ 0.01), which reduce to 3 I / R group the most significant. 6 rats RI / RI kidney tissue, HO-1 immunohistochemistry: HO-1 expression in renal tubular epithelial cells of the sham group was not obvious, HO-1 expression in the I / R group renal tubular epithelial cells compared with Sham group increased significantly compared with the I / R group, I / R, I / R and 3 I / R groups within the tubular epithelial cells HO-1 expression was significantly increased, which 3 I / R group of HO-1 expression the most obvious. Conclusions: 1 RI / RI, renal tubular epithelial cells was significantly impaired. RI / RI rats BUN, Scr, MDA, iNOS content than in the Sham group significantly increased SOD activity were significantly lower than Sham group were significantly increased renal ET, HO-1 immunoreactive particles increased expression in renal tubular epithelial cells . The 2 brief repeatedly IPC can significantly alleviate the RI / RI renal tissue injury, reduce BUN, Scr and MDA content, SOD activity increased renal tissue. Indicate that the brief repeatedly IPC can mitigate RI / RI rat kidney tissue damage and improve kidney function. 3 short-term repeated IPC can significantly reduce renal tissue iNOS content, to increase the content of NO reduce renal ET content, increasing the expression of HO-1 immune responses in the renal tubular epithelial cells positive particles. Show that the brief repeatedly IPC mitigate the RI / RI renal tissue injury mechanisms may be related to kidney HO-1 expression and increased NO content, reducing the content of ET and raised.
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