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Separation and Purification of Polyphenolic Bioactive Compounds from Rose Using Crosslinked Polymers

Author: MaiMaiTiMing·MaiHeMuTi
Tutor: TuErHong·MaiMaiTi
School: Xinjiang University
Course: Chemistry
Keywords: Molecularly Imprinted Polymers Crosslinked polymer Solid Phase Extraction Folin-Ciocalteu France HPLC method Polyphenol Microspheres Nevirapine
CLC: O631.3
Type: Master's thesis
Year: 2011
Downloads: 26
Quote: 0
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Abstract


Polyphenol metabolites is widely present in plants, because they have a wide variety of biological characteristics, has become a hot research and development today. Roses and pomegranate further development of medicinal value, and provide technical support for the separation and purification of plant polyphenols, we have synthesized several new types of solid-phase adsorption material combined with solid phase extraction investigate the selective solid-phase extraction plant extracts, polyphenols separation enrichment and other aspects of the application. Mainly includes: 1 Synthesis of a 1,2 - benzene diacrylate for Composite monomer, divinylbenzene crosslinker catechol acylating molecularly imprinted polymer; Synthesis of itaconic acid, methyl acrylic acid, acrylamide, styrene crosslinked polymer as the functional monomer, ethylene glycol dimethacrylate as a crosslinking agent. For solid phase extraction through leakage effect of the maximum adsorption capacity and optimum loading conditions when catechol concentration of 1.0 g · L -1 , pH = 2 sample buffer solution, the maximum adsorption capacity of the imprinted polymer reached 41.6 mg · g -1 ; optimization of the preparation conditions, the of itaconic acid crosslinked polymer adsorption reached 49.5 mg · g < sup> -1 . 2 by the solid phase extraction process optimization and using the Folin-Ciocalteu method, determined to IA-polymer of monomer synthesis using 0.1MHCl sample, water rinse, eluting with 95% methanol, the polymer having the best adsorption effect. The assay results show that Rose polyphenols in polymer equilibrium adsorption capacity of up to 55 mg · g -1 . Fitting can be obtained through the Langmuir and Freundlich adsorption isotherm polymer of polyphenol adsorption isotherm of Langmuir and Freundlich equation fits well. On the sample and leaching extract Vc different amount of recoveries from 98.2% to 102.4%; plant polyphenol content was measured by Folin-Ciocalteu method, and the correction due to the structural diversity of polyphenolic compounds, commercial The lack of standards and a number of structures not clarify and difficult to quantify. 3 using the suspension polymerization of a controlled particle size crosslinked microspheres IA? EGDMA, to examine the amount of dispersing agent, the two equal influence of the stirring speed and the oil / water polymerization system into the performance of the main factors of the ball and to determine the ball into the top condition. Using the Fourier transform infrared spectroscopy (FT-IR) to the chemical structure of the microspheres were characterized using a scanning electron microscope (SEM) observation of the morphology of the microspheres, the microspheres good sphericity, uniform particle size distribution. Appropriate conditions as follows: 10% of the amount of dispersant, compared to the stirring speed of 600 r / mim, oil / water 1:10. By BET analysis, polymers, resins D101, a specific surface area of ??the microspheres were: 214.43m2 / g, and the 144.039 m 2 / g, the 129.56 m 2 / g. 4. Valley a peak Act 275 nm and 299 nm wavelength at the absorption valley and peak guide number of amplitude as the measurement parameter, using first-order derivative ultraviolet spectral method determination of nevirapine concentration linear range of 2.5 ~ 25μg/mL, regression equation for D 275,299 = 1.4 × 10 -3 C 5 × 10 -5 , the correlation coefficient r = 0.9995, the average recovery rate was 99.46%. The first derivative spectrometry and RP-HPLC method is basically the same results, the accuracy and precision were no significant differences. Therefore, the first derivative spectrometry can be used for quality control of nevirapine tablets.

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CLC: > Mathematical sciences and chemical > Chemistry > Polymer chemistry ( polymer ) > Polymer physics and physical chemistry of polymers > The chemical nature of polymers
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