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Lung cancer is the most common malignancy in the world, the mortality rate in various types of tumors in the first place, its incidence has increased year after year. Non-small cell lung cancer accounts for more than 85% of lung cancer. Surgery, chemotherapy, radiation therapy, three means of treatment effect is still not satisfactory, tumor metastasis is the leading cause of death of patients with lung cancer. Epithelial - mesenchymal transition refers to the loss of epithelial markers, epithelial cells and the expression of mesenchymal markers, thereby obtaining the migration process. Many studies have found epithelial - mesenchymal transition is an important cause of tumor invasion and metastasis. The most significant change in the process of epithelial - mesenchymal transition of epithelial cells lost epithelial marker E-cadherin, beta-catenin expression of mesenchymal markers Snail, Vimentin. This study investigated the epithelial - mesenchymal transition-related protein E-cadherin, beta-catenin, Snail, Vimentin expression in non-small cell lung cancer and prognostic significance. Research methods. Collected from 165 patients with non-small cell lung cancer tissue paraffin blocks. Male 115, female 50 cases. The median age is 65 years old. Of which 107 cases of adenocarcinoma, 58 cases of squamous cell carcinoma. TNM staging of I 64 cases, II - IV of 101 cases. 165 patients with non-small cell lung cancer patients with complete follow-up data. 2. Complexes based on E-cadherin/β-catenin, Snail, Vimentin abnormal expression of the patients groups: group 1 (indicators showed no abnormality), group 2 (only one abnormal indicators), group 3 (two indicators an exception), group 4 (3 pointer exception), continue to be divided into: EMT-L1 group (group 1 and group 2), the EMT-L2 group (Groups 3 and 4). (3) the use of tissue microarray technology and immunohistochemistry EnVision two-step detection of E-cadherin, beta-catenin, Snail, Vimentin protein expression in non-small cell lung cancer. Grouped according to the situation analysis of the relationship between E-cadherin, β-catenin, Snail, Vimentin protein with clinicopathological data and prognosis. The results of E-cadherin, beta-catenin protein was located in the cell membrane, the positive expression rate was 66.7%, 50.9%. Snail protein was localized in the nucleus and Vimentin protein positive in the cytoplasm, the positive expression rates were 45.5%, 33.9%. Expression of E-cadherin and beta-catenin expression level was positively correlated (P = 0.003), and Snail protein expression levels were negatively correlated (P lt; 0.001). Of Snail expression in poorly differentiated tumor tissue, expressed in well-differentiated tumor tissue is relatively low, the expression difference was statistically significant (P = 0.013). Vimentin expression in tumor vascular invasion and no vascular invasion difference was statistically significant (P = 0.046) in cases with vascular invasion Vimentin expression is relatively high. Composite E-cadherin/β-catenin body, Snail, Vimentin abnormal expression group survival in patients were significantly lower than normal group (P = 0.0025, P = 0.0059, P = 0.0257). 3 composite body according E-cadherin/β-catenin, Snail, Vimentin abnormal expression level of these three proteins were divided into four groups, two or more abnormal expression of the group with no or only one abnormal expression of group of patients, the low degree of tumor differentiation (P = 0.003), and the relatively poor prognosis (P lt; 0.0001). Multivariate analysis showed that two or more abnormal expression is an independent risk prognostic factors. Conclusion 1. Patients with non-small cell lung cancer tumor cells in their epithelial cell marker E-cadherin, beta-catenin expression reduced expression of mesenchymal markers Snail, Vimentin, suggesting that tumor cells of epithelial - mesenchymal transition. 2, poorly differentiated tumor cell expression of Snail is relatively high, prompted Snail and the degree of tumor differentiation. Vimentin closely related to vascular invasion of cancer patients, suggesting that elevated expression of Vimentin can make tumor cells more susceptible to vascular invasion. 3, epithelial - mesenchymal transition the related protein E-cadherin/β-catenin complex, Snail, Vimentin any two proteins abnormal expression of low degree of tumor differentiation and poor prognosis patients with non-small cell lung cancer is closely related, could be used as non- independent prognostic indicators in patients with small cell lung cancer, a new molecular markers for prognosis and treatment of patients.
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