Dissertation > Excellent graduate degree dissertation topics show

Effect of 5-FU and ADM Combined with X-Ray Irradiation on Livin Expression and Apoptosis of MCF-7 Breast Cancer Cells

Author: ZhouTaoZuo
Tutor: ZhangXingPing
School: Chongqing Medical University
Course: Oncology
Keywords: Livin chemotherapeutics RT-PCR Immunohistochemistry Radiosensitization
CLC: R737.9
Type: Master's thesis
Year: 2011
Downloads: 19
Quote: 0
Read: Download Dissertation

Abstract


Objective: To observe 5-FU and ADM combined with X-ray irradiation interfering gene expression of Livin and apoptosis of MCF-7 breast cancer cells.Methods: The IC50 of 5-FU and ADM were detected by MTT in the time of 72 hours. The experiment had four groups: blank control group, X-ray irradiation group, 5-FU + irradiation group and ADM + irradiation group.The Immunohistochemistry and RT-PCR were used to evaluate the Livin protein and mRNA expression,and the apoptosis rate were analysed by flow cytometry.Results: Campare with the blank control group, the Livin protein and mRNA expression of X-ray irradiation group, 5-FU + irradiation group, ADM + irradiation group were reduced, and the apoptosis rate were increased (p<0.05). 5-FU and ADM combined with irradiation inhibited Livin protein and mRNA expression significantly compared with the X-ray irradiation group, and the apoptosis rate of the first two groups were increased. The difference were statistically significant (p <0.05). Conclusion: 5-FU and ADM combined with X-ray irradiation can enhance MCF-7 cell apoptosis, and with a radiosensitizing effect.The mechanism may be related to the down-regulation of Livin gene expression of MCF-7 breast cancer cells.

Related Dissertations

  1. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  2. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  3. The Study of Kisspeptin/GPR54 System on the Reproductive Regulation in the Hen’s Hypothalamus-Pituitary-Ovary Axis,S831
  4. Study on the Digestive Tube During the Embryonic Stage of Gushi Chicken,S831
  5. A Comparative Study of prawns, crabs, Spiroplasma disease immunohistochemistry and pathology,S945
  6. The Detection and Significance of CK 19 mRNA and MUC1 mRNA in Peripheral Blood in Cervical Cancer Patients.,R737.33
  7. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  8. Expressions of PRL-3 and p27 in Gastric Carcinoma and Their Clinical Significance,R735.2
  9. Expression of hnRNP A2/B1 and p21WAF1 Proteins in Lung Cancer and Their Clinical Significance,R734.2
  10. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  11. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  12. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  13. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31
  14. Expression and Clinical Significance of Survivin and YAP in Cervical Carcinoma,R737.33
  15. Expression and Clinical Significance of PTEN and Caspase-7 in Gastric Cancer and Precancerous Lesion,R735.2
  16. Cloning and Expression Analysis of NCCRP-1 AND IL-10 Genes in Grass Carp (Ctenopharyngodon Idellus),S917.4
  17. Expression and Antibody Preparation of sglt1 from Cyprinus CarpioL.,S917.4
  18. The Effect of Hepatic Denervation on Liver Regeneration after Partially Hepatectomy in Rats,R363
  19. Expression and Correlation of Caspase-3 and Cx43 in Myocardial Infarction with Different Narrow Degree Coronary Artery,R542.22
  20. The Expression and Relationships of Bmi-1 and c-myc in Diffuse Large B-cell Lymphoma Tissues,R733.1
  21. The Feasibility of the Immunohistochemistry Classification to Predicting Prognosis in Breast Cancer,R737.9

CLC: > Medicine, health > Oncology > Genitourinary tumors > Breast tumor
© 2012 www.DissertationTopic.Net  Mobile