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Effects of Ultra-High Pressure Treatment on the Structural Change of Tropomyosin of Bovine Skeletal Muscle

Author: WuYunNa
Tutor: GeRiLeTu
School: Inner Mongolia Agricultural University
Course: Agricultural Products Processing and Storage
Keywords: Extra high voltage Tropomyosin Surface hydrophobic groups Fluorescence intensity Mercapto group
CLC: Q51
Type: Master's thesis
Year: 2011
Downloads: 67
Quote: 1
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Abstract


In this study, extracted from bovine skeletal muscle tropomyosin , the purposes of 0.1 ~ 400MPa high pressure processing . Was measured after extracting tropomyosin aromatic surface hydrophobic groups, the surface of the aliphatic hydrophobic group , 280nm and 295nm excitation wavelength of the fluorescence intensity , changes in the spectral center of mass , light scattering properties , mercapto group content discussed pressure its structure . The results derived aromatic surface hydrophobic groups 100MPa when compared to the control group (0.1MPa) decreased the pressure continues to rise another upward trend . The hydrophobic group in the surface of the aliphatic 100MPa when compared with the control group was significantly reduced , and then slowly increased with the increase of the pressure to 400MPa Shiyou decreased slightly . 280nm and 295nm excitation wavelength tropomyosin fluorescence intensity downward trend with increasing pressure . The excitation wavelength of 280nm fluorescence intensity when the maximum fluorescence wavelength from 343.6nm move to 342nm, 295nm excitation wavelength from 343nm moving to 341 nm , the blue shift . Spectral center of mass 100MPa when compared with the control group , quickly reduced after decline slowly increasing pressure , the change was not significant between 100MPa and 200MPa and 300MPa and 400MPa . Light scattering intensity decreases with increasing pressure of 300MPa the lowest unzip recovery rate of 84.3 % , 400MPa when there are slightly higher recovery rate of 87.9% . Surface sulfhydryl content 100MPa when the atmospheric pressure , compared to an increase of 37.2% , and increased slowly between 100MPa to 400MPa . Derived from the above results , the pressure can cause changes in the structure of tropomyosin , and these changes may have a certain degree of reversibility .

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CLC: > Biological Sciences > Biochemistry > Protein
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