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Carbon nanotubes have unique mechanical, electrical and optical properties, making them unique materials for a wide range of potential applications, the potential applications of carbon nanotubes in the biomedical field, including biosensors, drug and vaccine carrier. While carbon nanotubes continue to develop a variety of potential applications, bio-safety studies of carbon nanotubes also caused widespread concern, one of the carbon nanotubes Biosafety of the important issues is how the carbon nanotubes with blood protein interactions. This paper studies a single-walled carbon nanotubes (SWCNT) adsorption of abundance in the blood, and the more important of the five proteins, including fibrinogen (BFg), immunoglobulins (γ-Ig), transferrin (Tf), albumin (BSA), and ferritin (ferritin). The fluorescence spectra quantitative analysis of the kinetics of adsorption of carbon nanotubes for these types of proteins found to reach equilibrium carbon nanotubes for protein adsorption a follows: BFg gt; gamma-Ig gt; Tf gt; BSA gt; ferritin . Electrophoresis (SDS-PAGE) found that the blood protein would compete adsorbed on the surface of carbon nanotubes and carbon nanotube protein adsorption capacity with fluorescent consistent. Protein adsorption capacity and the molecular weight of the protein on the carbon nanotubes, the number of hydrophobic amino acids was positively correlated with the atomic force microscope (AFM) imaging intuitive different protein adsorption mode in carbon nanotubes, the CD spectra show that the protein secondary The structure has undergone significant changes. Second, we studied the different structures, different sizes, different functionalized carbon nanotubes modified serum protein adsorption. By comparison, it is found that the important factors of the influence of carbon nanotubes on the protein adsorption ability is as follows: the structure of the carbon nanotubes, the arrangement pattern, size, and surface chemical modification. SDS-PAGE semi-quantitative analysis of carbon nanotubes on the adsorption of blood proteins, further confirms the previous conclusion. Types of carbon nanotubes adsorbed proteins identified by time-of-flight mass spectrometry (MALDI-TOF MS) found that the apparent adsorption in carbon nanotubes serum protein albumin, prealbumin, transferrin and immunoglobulin. Finally, the adsorption capacity of the serum protein by before and after comparison of different molecular weight polyethylene glycol (PEG)-modified carbon nanotubes, the PEG-modified carbon nanotubes on the adsorption of proteins can be suppressed, and inhibit the ability with the PEG chain structure and length. In short, this article about the study of carbon nanotubes on the adsorption of blood proteins made some important conclusions, these conclusions on an accurate assessment of the carbon nanotube bio-security is of great significance.
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