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Rho family proteins as Ras super family protein the most important member of the prevalence of fungi , and exercised important functions in their growth and development . Rho1 has occupied an important position in the Rho family of proteins , to understand Rho1 signaling networks in rice blast fungus , the research for the rice blast fungus interaction with Rho1 three proteins function . According to bioinformatic predictions , MoAca (Mgg 1 3499), MoCnd (Mgg 0 0256), MoPnrs (Mgg 0 9953) with Rho1 (Mgg 0 7176) interaction , the study by the yeast two-hybrid experiments verify the clear interaction of these genes Rho1 relationship . Study by homologous recombination , one by one to obtain these gene knockout mutants , and overexpression of MoCnd and MoPnrs mutants , and observe these mutant phenotypes , such as growth rate , sporulation , spore germination rate and pathogenic resistance changes. The results show that , compared with the wild-type strain , knock in addition the mutant body △ MoAca growth has slowed significantly , and its growth polarity also be appropriate inoculation experiments showed that weakened its pathogenicity . Knockout the the △ MoPnrs of the mutant phenotype in the growth rate , germination rate than the wild -type are not changed significantly , but significantly reduced sporulation , and its overexpression increased sporulation mutant phenomenon . Knock addition to the △ MoCnd of the mutant knock in addition to mutants and overexpression of the mutant phenotype compared with the wild-type had no significant change . In summary , part of the rice blast fungus interaction with Rho1 protein affects the vegetative growth of the rice blast fungus , pathogenicity and sporulation .
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