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Objective type distractor increased planting rabbit mandibular alveolar ridge to observe the process of distraction osteogenesis staphylococcus aureus injection role , and to explore its possible mechanism . Methods 40 healthy Japanese white rabbits weighing 2.0 ± 0.25kg, were randomly divided into experimental group and control group 2 groups of 20 , each group was divided into one day groups , respectively , for 1 week , 2 weeks and 4 groups week groups 5 . Adaptive feeding week later , the establishment of distraction osteogenesis model , after four days after the intermittent period, began to increase vertical rabbit mandibular alveolar ridge , 2 times / day , 0.5mm / second , a total of four days . Experimental group began the first day stretch , stretch after one week , two weeks , three weeks and four weeks , local injection in distraction staphylococcus aureus injection in the control group without drug . After different times gross observation , X-ray observation, histology , immunohistochemistry , and the average gray value measurement , measurement of serum calcium , phosphorus, and alkaline phosphatase (ALP) levels, and application software SPSS13.0 data were statistically analyzed. X-ray and histological results show after four weeks Distraction Distraction fixed gap basically completely filled with newly formed bone tissue . Experimental group at different times after bone metabolic activity , new bone formation and serum calcium, phosphorus , ALP content than the control group , and the difference in value between the two groups was statistically significant . Distraction after 1 day, 1 week , 2 weeks, 4 weeks , the experimental group and the control group immunohistochemical staining (BMP-2) between the average gray value difference was statistically significant (P lt; 0.01). These results showed that the experimental group compared with the control group , distraction bone metabolic activity and new bone formation were higher . Conclusion rabbit mandibular distraction osteogenesis , the local injection stretch zone staphylococcus aureus injection can achieve good bone regeneration , distraction can accelerate the process of new bone formation and reduce bone healing time .
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