Dissertation > Excellent graduate degree dissertation topics show

Helicobacter Infection and Its Impact on the Proliferation and Apoptosis of Human Esophageal Cancer Cell Line Eca-109

Author: LiChunYan
Tutor: JiangZhenYou；YeSiYing
School: Jinan University
Course: Pathogen Biology
Keywords: Esophageal carcinoma Helicobacter Cell proliferation Cell apoptosis proliferating cell nuclear antigen (PCNA) flow cytometry
CLC: R735.1
Type: Master's thesis
Year: 2009
Downloads: 35
Quote: 0
Read: Download Dissertation

Abstract


ObjectiveTo investigate whether there is an infection of Helicobacter in esophageal carcinoma and the influence of Helicobacter on the proliferation and apoptosis of human esophageal cell line Eca-109 cells in vitro experiment for exploration of Helicobacter infection in the significance of occurence of human esophageal carcinoma.Methods38 samples of esophageal carcinoma and 12 samples of normal esophageal tissues were randomly collected.Helicobacter-specific 16S rDNA were amplified with polymerase chain reaction(PCR).The target amplification products were sequenced and compared with 16S rDNA of H.pylori.published by GenBank.Then esophageal cancer cell line(Eca-109) was cocultured with sonicated extract of standard strain of H.pylori,which was followed by detections of cellular proliferation with MTT method,mRNA expression of proliferating cell nuclear antigen(PCNA) with semi-quantitative RT-PCR and cell apoptosis with flow cytometry.Results16S rDNA of Helicobacter species was found in esophageal cancer tissue in 34.2%(13/38) of patients with squamous carcinoma whereas none of the DNA of normal controls was detected.Four of Helicobacte-specific PCR amplicons were sequenced and compared with 16S rDNA of H.pylori published by GenBank in which 99-100%of homology was found. After Eca-109 was cocultured with different concentrations of sonicated extract of H.pylori, cell proliferation was obviously observed at low concentration of the extract.But Eca-109 cell apoptosis appeared at higher concentration.PCNA mRNA was increased compared with that of uninfected control.As demonstrated by flow cytometry,the sonicated extract of H.pylori induced the cell apopotosis with the rate increased in a concentration-dependent manner. ConclutionsHelicobacteraceae 16S rDNA are found in human esophageal carcinoma tissues which shows the infection of Helicobacter species likely H.pylori or other subspecies of Helicobacter.Helicobacter might be as one of the causes of contributing to generation and development of human esophageal carcinoma,in the mechanism of which the pathogen likely reproduces to a lower extent in esophageal mucosa and persistently lead to chronic inflammation resulting in persistent over expression of PCNA mRNA and unbalance between the cell proliferation and apoptosis.

Related Dissertations

  1. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  2. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  3. Proliferation and Differentiation Effects of Different Culture Methods and Cell Factors on Mouse Spermatogenic Cells in Vitro,R329
  4. Diagnostic Value of Multiple Tumor Marker Protein Biochip for Patients with Esophageal Carcinoma and Cholangiocarcinoma,R735.1
  5. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  6. Isolation and Cultivation of Rabbit Adipose-derived Stem Cells and the Influence of Platelet-rich Plasma on the Cell Proliferation,R329
  7. Inhibitory Role of Plasmodium Yoelii Circumsporozoite Protein on the Proliferation and Survial of Tumor Cell,R730.5
  8. Study on the Cell Cycle of Liver Cells during Rat Liver Regeneration,Q253
  9. Effects of Different Immunization on the Development of Immune Organs,cell Proliferation and Expression of Apoptosis-relatived Gene in Chicken,S858.31
  10. Epidemiology of Helicobacter Pylori Infection and Associated Factors among Workers in Dalian Port Group,R181.3
  11. Effects on Recombinant Human Interleukin-10 of IL-2, IFN-γ, GCs, T Cell Apoptosis in Transplantation of the Rabbit Skin,R392
  12. Down-regulation of ROCK Isoforms on Migration and Proliferation of Vascular Smooth Muscle Cells Stimulated by PDGF,R363
  13. The Expression and Clinical Significance of TH17 Cells Related Cytokines in the Patients with Rheumatoid Arthritis,R593.22
  14. Maximum Tolerated Dose Combined with Metronomic Chemoterpy on Proliferation and Angiogenesis in Treatment of Breast Cancer,R737.9
  15. Expression of rBTI-2 and Its Anti-tumor Activity,R730.2
  16. Effect of Ornithoctonus Huwena Venom on Proliferation of Human Colon Carcinoma Cell SW480 in Vitro,R735.35
  17. Helicobacter pylori infection on common complication of cirrhosis risk of,R575.2
  18. Expression of hPOT1、p53 and Their Relationship with HP Infection in Gastric Carcinoma,R735.2
  19. The Effect of Melatonin on Calcium Overload of Fetal Mouse Brain Cells Due to Lipopolysaccharide,R722.1
  20. Anti-tumor Effects of Lupeol at Different Regimens and Its Role in Immune Systems,R730.5
  21. Intense Pulsed Light in Effects of TGF-β1 Induced on the Proliferation of Fibroblasts and Intervention of ERK Inhibitor,R751

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Esophageal tumors
© 2012 www.DissertationTopic.Net  Mobile