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The Reverse Effect of Brucea Javanica Oil Emulsion on Drug-resistant Human Ovarian Carcinoma Cell Line A2780/DDP

Author: ChenDan
Tutor: ChenPing
School: Hubei College of Traditional Chinese Medicine
Course: Traditional Chinese Medicine
Keywords: Javanica oil emulsion Ovarian Cancer A2780/DDP cells Resistance reversal
CLC: R737.31
Type: Master's thesis
Year: 2009
Downloads: 187
Quote: 1
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Abstract


Objective To observe the javanica oil emulsion on human ovarian cancer resistant cell lines A2780/DDP in drug reversal effect, to explore its resistance reversal mechanism of ovarian cancer, to provide experimental evidence for resistance reversal effect javanica oil emulsion used in clinical . Methods MTT assay sensitivity of human ovarian carcinoma parental cell line A2780 and resistant cell lines A2780/DDP cisplatin and javanica oil emulsion, to calculate the rate of cell growth value × 100% = the absorbance values ??of the experimental group / control group absorbance experiments repeated three times and averaged the results of drug concentration as abscissa to the growth rate of the ordinate, the concentration of the drug - the growth rate curve drawn through Excel software, calculated by the IC 50 calculation software of drug action 50% cell survival after 48h drug concentration (IC 50 ), resistance index (RI) = IC 50 (A2780/DDP) / IC 50 (A2780). Cisplatin on two cells the Department A2780/DDP and A2780 IC 50 and RI reversal agents (not plus) is calculated in accordance with the above method. Non-toxic dose of IC javanica oil emulsion method 98 . The concentrations in A2780/DDP javanica oil emulsion in IC 98 , cultured 24h, were seeded in 96-well plates, the MTT assay cisplatin cell lines A2780/DDP IC 50 and RI '(reversal agents). And calculate the the resistance reversal multiples RI / RI '. FCM detect A2780/DDP apoptosis, were divided into four groups: control group (control group), javanica oil emulsion group (traditional Chinese medicine group), cisplatin group (western medicine group), javanica oil emulsion cisplatin group (Western medicine group), each group of three parallel samples, 25cm 2 of culture flasks inoculated with 1 × 10 5 cells. Collection of groups treated cells fixed, stained, flow cytometry apoptosis, with Multicycle software analysis. Immunofluorescence cytochemical method (operation) according to kit instructions detect blank group, javanica oil emulsion group, DDP group, javanica oil emulsion cisplatin group drugs after A2780/DDP cells Bcl-2 expression changes. Western blot method (operation) according to kit instructions detect blank group javanica oil emulsion group, cisplatin group javanica oil emulsion cisplatin group the drug action A2780/DDP cells MRP expression changes. 1 javanica oil emulsion growth inhibition of human ovarian cancer cell lines A2780/DDP enhanced with the increase of the concentration of the drug. 2.A2780/DDP by 0.02g L -1 javanica oil emulsion after 24h resistance reversal multiple of 5.49 times (5.6/1.02). Javanica oil emulsion the reversal ovarian cancer resistant strain A2780/DDP resistance role. Blank group javanica oil emulsion group, cisplatin group, javanica oil emulsion cis-platinum group after 24h of drug action apoptosis rate were 1.98%, 2.11%, 3.15%, 10.3%, and the other group alone drug resistant cells compared javanica oil emulsion cis-platinum group resistant to apoptosis was significantly improved. Immunofluorescence method to detect the Bcl-2 protein expression compared with other separate groups use drug resistant cells javanica oil emulsion cis-platinum group resistant cells Bcl-2 protein expression was significantly reduced, and results show that the Brucea javanica oil emulsion to reduce the expression of Bcl-2 (P <0.05). 5.Western blot assay groups MRP protein alone group with other drug-resistant cells compared javanica oil emulsion cisplatin group resistant cell MRP protein expression was significantly lower results show that the javanica oil emulsion can reduce the MRP expression. Conclusion 1. Javanica oil emulsion on human ovarian cancer cell lines A2780/DDP has resistance reversal effect. Javanica oil emulsion mechanisms of human ovarian cancer cell lines A2780/DDP resistance reversal effect may be related to promotion of the resistant cell apoptosis, reduced the expression of Bcl-2 protein and MRP.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Ovarian tumors
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