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ObjectiveThe main purpose of this task is to optimize the best parts of Ginkgo biloba extract effective technology, and preparation of Ginkgo flavonoids injection, and establish quality standards, to meet the quality standards of injection.MethodsChinese herbal medicine ginkgo biloba side, UV Spectrophotometric Determination of total flavonoids in the leaves, through the orthogonal experiment, the concentration of ethanol, reflux temperature, extraction time factors, each of the three levels of the election, with ethanol extraction extraction, optimization of extraction conditions; with AB-8 macroporous resin purification bold things, through experiments eluent to optimize elution conditions. Injection molding study, by examining the solution PH value and the amount of activated carbon, ultraviolet spectrophotometry injection of total flavonoids in Ginkgo biloba, and were to inspect each other’s jurisprudence. Injection with the TLC of Ginkgo biloba in medicine there.ResultsGinkgo biloba extract rate of 60% ethanol, reflux temperature of 80℃, extraction time was 3h. Concentrated to 0.84mg.crude drug ? mL-1, with AB-8 macroporous adsorption resin treatment, the first resin with 2 times the volume of water for injection then eluted using three times the resin volume of 30% ethanol elution, collection of ethanol concentration lotion to 0.84mg crude drug ? mL-1, filling water injection 50ml, 450ml of ethanol dissolved; and stir slowly adding 4% gelatin to precipitate no longer up, standing 30min, shop with sterile double-sterile Büchner funnel filter paper filtration; filtrate to pass the inspection after tannin at 60℃command to no alcohol taste; for an alternative acetic acid 0.98ml, 1.8g sodium acetate plus water for injection 100ml dissolved, add to them, add water to 300ml; joining forces with sodium 0.5g, Stir dissolved; plus alcohol 25ml, 1,2 propylene glycol 25ml, add to the total amount of water for injection 500ml. Adding pin activated carbon 0.25g(2.5‰), 60℃under the sand core circle 20min, removing carbon, 0.45μm membrane filter. Filtrate after passing inspection, each mounted volume by filling in 5ml 5ml ampoules in nitrogen filling, sealing. 115℃Heat sterilization 30min, which was Ginkgo flavonoids injection.TLC injection of Ginkgo biloba in the presence of herbs, the results show that specific, reproducible, and no interference with the negative control. Ginkgo Ginkgo flavonoids injection determination of total flavonoids according to UV experiments to absorbance (A) on the concentration (C) (mg ? ml-1) Plot the standard curve, absorbance measured at 504nm wavelength and recorded absorbance values, standard curve generation, that is to get ginkgo flavonoids.ConclusionsGinkgo flavone injection preparation reasonably practicable, quality control, product stability.
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