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It has been clear that the FT gene in Arabidopsis photoperiodic induction of flowering pathway plays a key role . In this study, the model plant Arabidopsis ft-10 mutant and wild-type material , by means of two-dimensional electrophoresis and mass spectrometry experiments to study the differences in the level of both protein expression , help -depth study of FT regulation photoperiod flowering mechanisms. The results are as follows: (1) by protein lysate optimize plant protein extraction and matching , filter out a suitable bidirectional Arabidopsis leaves protein analysis electrophoresis sample preparation and analysis system : ammonium acetate / methanol precipitation leaf protein samples lysis buffer [ 8mol / L urea , 2 mol / L thiourea , 4% CHAPS , 60mmol/LDTT 2% Ampholine , 1 mmol / L PMSF ] cracking 1.5mg on samples after test after transfection background clear two-way , high-resolution protein electrophoresis pattern . (2) In this study, Arabidopsis ft-10 protein with wild-type leaves contrast to the two-dimensional electrophoresis analysis of differential protein spots were digested mass spectrometry, MALDI-TOF MS analysis of the final for the purposes of 34 protein spots and database searches identified a total of 26 proteins have been identified , protein identification success rate of 76% . Have identified the protein , ft-10 mutant body compared with the control , 16 protein spots were up-regulated (point 3,5,6,10,11,12,15,16,17,18,20,23,24 , 25, 30, 35 ) ; expression level of 11 protein spots to cut (point 4,7,8,9,14,22,26,28,32,33,34 ) ; possible for newly synthesized proteins ( points 21, 27 ) . They belong to the transcriptional regulation of plant defense -related signal transduction , energy transfer , storage proteins , structural proteins , cell growth , and metabolism -related enzymes or proteins such as protein types .
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