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Established by this experiment, extracted from beeswax Octacosanol the Octacosanol O / W emulsion method of preparation, while the impact of the different food additives on the stability of the emulsion were studied in detail. Extractant and its dosage, extraction temperature, extraction time and extraction with distilled water as the extraction influencing factors through single factor test, explore and determine the the Octacosanol optimal extraction parameters, being drawn by the experimental results of the single factor cross test level factors. Based on orthogonal experiment and variance analysis to determine the optimal extraction conditions: the extractant select 120 # gasoline, the extraction time of 50 min, extraction temperature of 70 ° C, the amount of 150 mL extractant distilled water, the amount of 75 mL can be obtained 42.89% of the crude product, which octacosanol content of 15.35%. 2, recrystallization solvents, solid-liquid ratio, temperature, number of crystallization the Octacosanol purified single factor test, when the crude alcohol extraction solvent solid-liquid ratio of 1:1 15, re-crystallization temperature of 20 ° C, the solvent is chloroform / n-hexane (3:1), and the crystal 6 times, you can obtain a purity of 57.28% octacosanol. Crystallized three times Octacosanol content increases rapidly with the increase in the number of crystallization, the Octacosanol content also increased, but more much slower. The trials also proved recrystallized if the choice of two kinds of solvents used interchangeably way, i.e. the first crystallized with n-hexane three times, and then chloroform was crystallized twice, and finally then hexane crystallized twice, can be obtained in more than 80% pure, but Octacosanol yield is very small. 35 ° C several crystals: the Octacosanol the Octacosanol content in the crystals and the filtrate content decreases with crystallization of the increase in the number of possible causes Octacosanol higher than octacos Advanced alkanol harder to precipitate crystals in the crystallization conditions, i.e. more easily into the filtrate. Accordingly, crystallization may be several times through 35 ℃ effective separation of the high-level higher than that in the crystalline material is removed octacos alkanol. 3, using an oil-soluble emulsifier Span 80 and a water-soluble emulsifier Tween 80 mixed Octacosanol can make a water-insoluble to form an O / W emulsifier, 0.6% xanthan gum, 0.2% CMC 0.4% gum arabic complex stabilizers may be the emulsion has good stability, and emulsified under high shear speed of 15 × 103 r / min for 25 min, through a pressure 30 to 35 Mpa, a temperature of 60 ~ 70 ℃ homogenizer 2 times the Octacosanol emulsion stability can be obtained. Octacosanol emulsion as a pale milky white liquid, lighter taste emulsifier (25 mL/1000 mL), the less the amount added in the preparation of the sports drinks, and does not impact on the taste of the beverage and transparency. A low concentration of monovalent salt, a divalent salt the Octacosanol emulsion stability increased, decreased stability at high concentrations; same sugars, organic acids at low concentrations, the octacosanol emulsion The stability increased, the decrease in the high concentration.
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