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Stage-specific Proteomics and Metal Elements Analyses from Zygotic Embryo and Female Gametophyte Tissue of Chinese Fir (Cunninghamia Lanceolata (Lamb.) Hook)

Author: XuShuPing
Tutor: ShiJiSen
School: Nanjing Forestry University
Course: Biochemistry and Molecular Biology
Keywords: Chinese fir Zygotic embryos Female gametophyte Protein Metal elements
CLC: S791.27
Type: Master's thesis
Year: 2009
Downloads: 36
Quote: 1
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Abstract


Fir (Cunninghamia lanceolata (Lame.) Hook) is our unique evergreen conifer species , distributed in the south of the Yangtze River Basin , rapid growth , material quality , value ranks the forefront of important timber species . Currently , somatic embryogenesis become a potentially important means to achieve large-scale production of the fir . In this study, based on similarity zygotic embryos and somatic embryos during development from two aspects of protein levels and metal elements to study the the zygotic embryo 's development mechanism and to provide the basis for further optimization of somatic embryogenesis technology exploration . 153 protein spots of interest , image analysis , based on 2-D DIGE protein expression profiles of fir zygotic embryo and female gametophyte . The cotyledon stage high expression of protein mass spectrometry results show that these proteins are associated with the zygotic embryo development process storage class protein , single dehydroascorbate reductase small heat shock protein , duration of protein . Fir zygotic embryo and female gametophyte metal elements by inductively coupled plasma emission spectrometry analysis of the results are as follows : the content of Cu in the process of development of the zygotic embryos showed a slight upward change ; amount of P 's overall showed a big increase ; K , Mn , Ca the amount decreased first , upward change ; Mg late embryo development in the body continued to rise , then the highest value ; lower the content of Co and Ni , the content of Co is not more than 1μg / g , the Ni content of not more than 3μg / g .

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CLC: > Agricultural Sciences > Forestry > Forest tree species > Coniferous > Chinese fir
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