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The Clinical Application Research of Detecting the Molecular Cytogenetics Mutation of Bladder Neoplasms with Fluorescence in Situ Hybridization

Author: ZhangXiaoLei
Tutor: SongXiShuang
School: Dalian Medical University
Course: Urology
Keywords: Bladder neoplasms Fluorescence in situ hybridization Cytogenetic Diagnosis
CLC: R737.14
Type: Master's thesis
Year: 2009
Downloads: 84
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Abstract

Objective: Bladder neoplasms is the most common malignant tumor of the male reproductive system in our country, although the majority of bladder neoplasms can be treated through surgery, but it makes the high recurrence rate of bladder neoplasms, how to make early diagnosis and effective prevention of the recurrence of bladder neoplasms has become Urology urgent important issues. The purpose of this study using FISH groups to explore Chinese chromosomal abnormalities and bladder neoplasms occurrence and development of the relationship, through the experimental set up in line with the characteristics of the Chinese molecular cytogenetics data of bladder neoplasms, for the adoption of the exfoliated cells in urine early diagnosis of bladder neoplasms and monitoring the prognosis of bladder neoplasms to provide a new way to bladder neoplasms in Chinese will be non-invasive, non-painful and high specificity, high sensitivity method for early diagnosis and provide a theoretical basis.Methods: Collected 100 cases of patients with hematuria and 20 healthy control group of fresh urine, simultaneously in urine exfoliated cells morphological examination and FISH detection of 3rd and 7th chromosome. 100 cases of hematuria in the urine of patients with cancer cells to find and identify the cells in urine exfoliated cells of the mor- phological examination, the sensitivity and specificity. FISH to determine: two red signals and two green signals are for normal cell signal, one signal or more than three abnormal signals are abnormal cell signal. Healthy control group of urine fluorescence in situ hybridization detection of abnormal signal point to determine the value of the domain, and then through the urine of patients with fluorescent in situ hybridization, FISH methods identified in the detection of bladder cancer patients with sensitivity, specificity . Comparison of two methods, obtained through the Statistics test P value, P<0.05 for significant differences exist.Result: FISH using probe CSP3/CSP7 healthy control groups in urine exfoliated cells, abnormal signal point value of the domain: CSP3 probe: a signal at 4.1%, the signal of 3 and more than 3 points domain value of 5.8%; CSP7 probe: a signal at 4.3%, 3 and 3 points above the signal threshold of 4.9%.①, Domain value in the use of FISH detection of hematuria in urine exfoliated cells of patients with a sensitivity of 84.5% and specificity of 77.8%. Exfoliated urothelial cells and the cell morphology of the sensitivity of 61.5% and specificity of 83.3%. Obtained through theχ2 test P value, the results of FISH testing of urine exfoliated cells and cell morphology of the positive rate and there are significant differences in sensitivity (P<0.05), specificity was no significant difference (P>0.05). FISH detection than that of urine exfoliative cells positive rate of morphological examination and a higher sensitivity and specificity considerable.②, FISH detection of bladder neoplasms of the sensitivity of different pathological stages Ta period for 78.6%, T1 period of 88.4%,> T2 period of 89.5 percent, was gradually increasing trend, but statistical analysis showed that the phases was not significant between differences (P> 0.05). FISH testing for bladder neoplasms the sensitivity of different stages of non-discrimination.Conclusion: Exfoliated urothelial cells for FISH testing is an effective diagnosis of bladder neoplasms detection methods. It is used for the diagnosis of bladder neoplasms with high sensitivity and specificity, and non-invasive, non-painful characteristics of exfoliated cells and urine of cell morphology contrast, the higher sensitivity and specificity was no significant difference. For the early diagnosis of bladder cancer has pro- vided an effective new way. Chinese but also for non-invasive bladder cancer, non-painful and high specificity, high sensitivity method for early diagnosis provides a theoretical basis.

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