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Purpose : the chondrocytes NO observed cyclical tensile strain induced by rhIL-1β and MMP-3 synthesis and IL - mediated chondrocyte glycosaminoglycan synthesis inhibition antagonism to understand the mechanical signal in cartilage repair activities mechanism. : Cartilage cells were isolated and cultured in vitro culture of the rabbit knee P1 generation chondrocytes were randomly divided into five groups , each consisting of 12 samples were divided into control group , IL-1β (Interleukin-1β, IL-1β) ( 1ng/ml) alone group and IL-1β and cyclical tensile strain (cyclic tensile strain, CTS, 3%, 0.25Hz, sine wave , CTS role together groups of 8, 16 and 24 hours) , 24 hours after receive each group cultured cell supernatant was measured MMP -3 , NO and glycosaminoglycan content , comparing each group changes . Results : normal rabbit knee the the P1 generation cartilage cells secrete a certain amount of MMP- 3, NO, IL - 1β action group MMP -3 , the content of NO was significantly higher , the difference was statistically significance ( P lt ; 0.001 and P lt ; 0.05 ); IL-1β and CTS together group of MMP -3 , the content of NO was significantly reduced compared with the role of IL-1β group , the differences among the groups with statistical significance ( P lt ; 0.001 and P lt; 0.05 ) , the CTS role 8h best block the synthesis of MMP-3 and NO . Glycosaminoglycans , the role of IL -1β group glycosaminoglycan content was significantly reduced , and the difference was statistically significant ( P lt; 0.001 ) ; significantly higher IL -1β and CTS joint action group glycosaminoglycan content with IL-1β role the differences between groups were statistically significant ( P lt; 0.001 ) compared to 16 hours in the CTS block glycosaminoglycan synthesis inhibition caused by IL-1β . Conclusion : The cyclical tensile strain reduce IL-1 -induced rabbit articular chondrocytes NO . , And MMP-3 synthesis in vitro , and block the IL-1 -mediated inhibition of glycosaminoglycan synthesis . Cyclical tensile strain may be provided by inhibiting the inflammatory reaction of NO and of MMP-3 production and promote the synthesis of glycosaminoglycans in the inflammatory response involved in OA cartilage damage repair process, for the role of the continuous passive motion in OA therapy provides a certain experimental data .
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