Dissertation > Excellent graduate degree dissertation topics show

Study on Different Expression of PPAR-β in Human Hypertrophic Scar and Normal Skin Tissue

Author: HuangYun
Tutor: YangXingHua
School: Central South University
Course: Surgery
Keywords: PPAR-β fibroblasts cells hypertrophic scar RT-PCR immunohistochemistry
CLC: R622
Type: Master's thesis
Year: 2011
Downloads: 7
Quote: 0
Read: Download Dissertation

Abstract


Background:Hypertrophic scar was pathological scar mainly caused by trauma which injured to dermis characterized by the excessive proliferation of fibroblasts and extracellular matrix excessive production and deposition. Hypertrophic scar could cause significant defects in appearance or dysfunction depended on different growth position. Hypertrophic scar was the major problem which trauma patients needed to face during post-rehabilitation. The prevention and cure of hypertrophic scar was always a difficult matter but we were not clear on the mechanism of hypertrophic scar so far. PPAR-βwhich belonged to PPARs family was rich in skin. The research showed that PPAR-βwas important to the process of wound healing. Hypertrophic scar was considered to be the result of excessive wound healing, but the studies on PPAR-βin the formation and development of hypertrophic scars was few.Objective:To compare the different expression of PPAR-βbetween human hypertrophic scar and normal skin tissue. To provide a new research direction on the formation and development of hypertrophic scar and a new strategy of curing hypertrophic scar. Methods:The specimen of hypertrophic scar and the corresponding normal skin tissue was obtained from 16 patients underwent scar surgery from January 2010 to January 2011 in Burns and Plastic Surgery of Xiangya hospital of Central South University. RT-PCR was performed for semi-quantitative analysis of the expression of PPAR-βmRNA in hypertrophic scar and normal skin. Immunohistochemistry was performed to determine the expression and localization of PPAR-βprotein in the hypertrophic scar tissues and to further verify the different expression of PPAR-βin tissueResults:RT-PCR analysis result indicated that PPAR-βmRNA was expressed in hypertrophic scar and normal skin tissue but the expression level of PPAR-βmRNA was higher in hypertrophic scar tissues than that in normal skin tissue with significant difference (t=2.361,P=0.028). The result of immunohistechermical analysis indicated PPAR-βprotein mainly located in the cytoplasm of fibroblasts cells and there were 12 patients with PPAR-βpositive expression in hypertrophic scar specimens (positive rate 75%) compared that 5 patients in normal skin tissue specimens (positive rate 31.2%). the expression level of PPAR-βprotein was higher in hypertrophic scar tissue than that in normal skin tissue with significant difference (X2=6.14 P=0.013)Conclusion:We firstly demonstrated the higher expression of PPAR-βin hypertrophic scar tissues than normal skin tissues with significant difference. Our results indicated that PPAR-βmay play an important role in the formation and development of hypertrophic scar

Related Dissertations

  1. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  2. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78
  3. Isolation, Identification and Molecular Characteristics of Japane Encephalitis Virus in Henan Province,S852.65
  4. Studies on the Difference of PRRSV Infectious and PRRSV ADE,S858.28
  5. cDNA-cloning and Expression analysis of Phosphate Stress Induced Purple Acid Phosphatase Genes and transcription Factor Genes from Seeding Wheat,S512.1
  6. Study on Key Technology and Its Physiological Basis of Virus Elimination and Rapid Propagation of Phalaenopsis,S682.31
  7. Molecular Mapping of Downy Mildew Resistant Gene and Expression of Rs-AFPs in Radish (Raphanus sativus L.),S631.1
  8. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  9. The Study of Kisspeptin/GPR54 System on the Reproductive Regulation in the Hen’s Hypothalamus-Pituitary-Ovary Axis,S831
  10. Study on the Digestive Tube During the Embryonic Stage of Gushi Chicken,S831
  11. A Comparative Study of prawns, crabs, Spiroplasma disease immunohistochemistry and pathology,S945
  12. Proliferation and Differentiation Effects of Different Culture Methods and Cell Factors on Mouse Spermatogenic Cells in Vitro,R329
  13. The Detection and Significance of CK 19 mRNA and MUC1 mRNA in Peripheral Blood in Cervical Cancer Patients.,R737.33
  14. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  15. Expressions of PRL-3 and p27 in Gastric Carcinoma and Their Clinical Significance,R735.2
  16. Expression of hnRNP A2/B1 and p21WAF1 Proteins in Lung Cancer and Their Clinical Significance,R734.2
  17. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  18. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  19. Effect of HCO3-, K+ and HSO3- on rbcL and rbcS Gene Expression and Photosynthesis of Soybean Seedlings,S565.1
  20. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  21. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31

CLC: > Medicine, health > Surgery > Plastic Surgery (repair surgery ) > Plastic surgery school
© 2012 www.DissertationTopic.Net  Mobile