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Objective To investigate the children in Guangxi Nanning, Liuzhou and Baise incidence of α-thalassemia (α-thalassemia), genotype and gene frequencies, and compare the difference in the three regions and different ethnic. Stratified cluster random sampling method to select a total of 1789 half of children aged 3-6 in the three regions of Guangxi Nanning, Liuzhou, Baise, venous blood, ACD anticoagulant, blood test, hemoglobin electrophoresis and high performance liquid chromatography analysis hemoglobin quantification. The polymerase chain reaction (polymerase chain reaction, PCR) and reverse dot blot hybridization detection of α-thalassemia gene mutations. Application SPSS 13.0 statistical software for statistical processing of data. 1, the subject selected in 1789 half of children aged 3-6 in the three regions of Guangxi, were detected in 224 cases of alpha thalassemia, alpha thalassemia incidence of 12.52%. Has detected 224 cases of α-thalassemia: ① light alpha thalassemia, a total of 130 patients (58.03%), including: Southeast Asian deletional α thalassemia (--SEA/αα) of 124 cases (55.36%), Thailand deletion α thalassemia (--Thai/αα) of 2 cases (0.89%), homozygous -3.7 deletion α thalassemia (-α3.7 /-α3.7) and 2 cases (0.89%), homozygous -4.2 deletion α thalassemia (-α4.2 /-α4.2) 1 (0.45%), double heterozygotes (-α4.2 /-α3.7) 1 (0.45%). (2) static type α-thalassemia, 85 cases (37.95%): -3.7 deletion α thalassemia (-α3.7/αα) of 41 cases (18.30%), -4.2 deletion α thalassemia (-α4.2/αα ) of 19 cases (8.48%), non-deletional Hb Constant Spring mutation (αCSα / αα) of 24 cases (10.71%), non-deletional Hb Quong Sze mutation (αQSα / αα) 1 Li (0.45%). ③ Hb H disease in 9 cases (4.02%): Southeast Asian deletion and -3.7 deletional α-thalassemia heterozygotes (--SEA/-α3.7) 3 (1.34%), Southeast Asia deletion and -4.2 deletion α thalassemia heterozygotes (--SEA/-α4.2) of 2 cases (0.89%), Southeast Asian deletional α thalassemia and non-deletional Hb CS heterozygotes (--SEA/αCSα) 4 (1.79%) . 3, Nanning, Liuzhou and Baise α Mediterranean anemia detected in a number of cases and incidence rate: Baise, 93 cases (16.15%), Nanning, 48 cases (10.91%), Liuzhou, 83 cases (10.74%). , Nanning, Liuzhou and Baise Han, Zhuang α-thalassemia detected in the number of cases and incidence rate: Han detected in 71 cases (0.09%), Zhuang detected in 121 cases (0.13%). 5, Nanning, Liuzhou and Baise area boys, girls, α-thalassemia detected in a number of cases and incidence rate: boy detected in 128 cases (13.06%), the girls were 96 cases (11.87%). Conclusion 1, 1789 Children of Guangxi Nanning, Liuzhou and Baise alpha thalassemia rate of 12.52%. , Guangxi Nanning, Liuzhou and Baise children mainly to --SEA/αα, α-thalassemia genotype order: -α3.7/αα the, -α4.2/αα αCSα / αα - SEA / αCSα, --SEA/-α3.7, --SEA/-α4.2,-α3.7 /-α3.7, --Thai/αα,-α4.2 /-α4.2,-α4 .2 /-α3.7, αQSα / αα. 3, children in Guangxi Nanning, Liuzhou and Baise common genotype --SEA/αα, -α3.7/αα -α4.2/αα αCSα / αα; rare genotype --Thai/αα, αQSα / αα. , Nanning, Liuzhou and Baise Zhuang alpha thalassemia children a high incidence of the Han children; Zhuang Children genotype species than Han children. Prompted Zhuang Children incidence of α-thalassemia and complexity are higher than the Han Chinese children. Baise region of α-thalassemia was higher than in Nanning and Liuzhou. 6, was first detected in the domestic children rare Thai deletional α-thalassemia 1.7, the results provide the basis for genetic counseling of these areas and children's future marriage and child rearing guidance.
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