Dissertation > Excellent graduate degree dissertation topics show

Cloning and Sequence Analysis of Hr and RORα Gene of Goat

Author: LuHongYan
Tutor: ZhangWenGuang；LiJinQuan
School: Inner Mongolia Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Goat Hr RORα Sequence analysis
CLC: S827
Type: Master's thesis
Year: 2010
Downloads: 25
Quote: 0
Read: Download Dissertation

Abstract


Some mammalian species appear partial or complete absence of hair at birth. The Hr gene is often responsible for this disorder. In addition, the product of the thyroid hormone-regulated mammalian gene Hr was recently show to function as a thyroid hormone receptor corepressor of RORα.Here we cloning and sequence analysis of the primary structure,base compositionand amino acids composition of Hr and RORαof goat and to alay the foundation for research the molecular mechanisms between the two gene.We got Hr DNA sequences about 9610bp,consist of 14 exons(9 exons integrated) and 11 introns.Sequence analysis revealed that Hr nucleotides sequences were high degree of conservation with sheep and cow,the similarity are 98.6% and 96.6%. Hr has abundance of polymorphism.We found 785 loci were polymorphism and accounted for 39.8% .And we found that in sheep,cow and goat Hr exon3,there are 9 bases less than other mammalian species.The sequence is GGGGTCTTG,translate Gly,Gly and Leu. We got RORαDNA sequences about 3925bp,consist of 2 exons and 1 intron.Sequence analysis revealed that RORαnucleotides sequences were high degree of conservation with cow,the similarity is 97.9%. compare with other species ,the conservation all surpass 90%. RORαhas poor polymorphism,we only found 57 loci were polymorphism and accounted for 15.3%.

Related Dissertations

  1. Survey in Nanjing and Analysis of the Mitochondrial Genome of Frankliniella Occidentalis (Pergande),S433
  2. Isolation and Identification of Low Pathogenicity Avian Influenza (H9) Virus in Henan and Study on the Biological Charactistics,S852.65
  3. Isolation and Identification of Infectious Bronchitis Virus and Sequence Analysis of Its S1 Gene and N Gene,S852.65
  4. Pathogenic Mechanism of Invasion Associated Genes in Avian Pathogenic E. Coli Duck Isolate DE205B,S852.61
  5. A Role of Auxin in Repression of Harpin-Induced Hypersensitive Response in Tobacco,S432.1
  6. Cloning 1Dx5 Gene from the Xinjiang Wheat Variety and Constructing Its Expression Vector,S512.1
  7. Mitochondrial Genome Sequence Analysis of a Brassica Juncea Landrace,S565.4
  8. Effects of Two Kinds of Zinc Form on Expression of Zinc Transporter Gene SLC39A1 to 3 in Dairy Goat,S827
  9. Molecular Epidemiology about White Spot Syndrome in Jiangsu Province,S945.1
  10. Cloning, Expression and Bioactivity Analysis of Goat Interleukin-1β and Interleukin-6,S827
  11. Introduction of Goat Parents and Its Feeding Technique Improved in Southern Zhejiang Mountainous Area,S827
  12. Design and Realization of Human Resource Management System,TP311.52
  13. The Relationship between ghrelin and High-fat Diet-induced Obesity in Rats,R589
  14. Study on the Strategy of LD’s Development in Wuhan,F249.2
  15. Based on human resource capability maturity Training System A department store,F721
  16. NJ RCCs Human Resource Management Assessment and Reform,F832.35
  17. Isoquinoline alkaloids SIPI409 Antiarrhythmic effect of,R965
  18. Isolation and Identification of NDV Local Epidemic Strains and the Comparison of Vaccination Programs,S852.65
  19. Establishment of a Nested PCR Assay and Analysis of the Full Genome of HUMAN COSAVIRUS,S855.3
  20. Investigate the Levels of CD4~+CD25~+CD127~- Regulatory T Cells in Peripheral Blood of Patients with Cervical Carcinoma,R737.33
  21. Research and Implementation of Human Resources Dispatch in Software Enterprises,TP311.52

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Goat
© 2012 www.DissertationTopic.Net  Mobile