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Immunologic Defence of Astaxanthin to Litopenaeus Vannamei and Water Quatity Monitoring WSSV Detecion in Shrimp Culture

Author: WangHuiChun
Tutor: GuanYueQiang
School: Hebei University
Course: Hydrobiology
Keywords: Litopenaeus vannamei Astaxanthin Immune protection White Spot Syndrome Virus ( WSSV ) Antioxidant enzyme gene Water Quality in
CLC: S968.22
Type: Master's thesis
Year: 2010
Downloads: 128
Quote: 2
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Abstract


Astaxanthin Add to Litopenaeus shrimp ( Litopenaeus vannamei ) , starting from the perspective of antioxidant compound feed , individual, cellular and molecular levels of astaxanthin impact on the Litopenaeus vannamei protective immunity and antioxidant enzyme gene , a comprehensive evaluation of shrimp astaxanthin in improving Litopenaeus vannamei physiological functions and improve Litopenaeus vannamei disease resistance and other aspects of the role , and then speculated that the mechanism of action of astaxanthin . Astaxanthin immune protective effect of L. vannamei infected with WSSV . Evaluation indicators : blood immune parameters ( blood count, blood cell phagocytosis percentage of phagocytic index , serum antibacterial activity , serum lysozyme activity , serum phenol oxidase activity , serum superoxide anion radical activity ) , cumulative mortality of WSSV infection and relatively immune protection rate . Proved: 80 mg / kg astaxanthin can significantly improve the hematological parameters of the experimental group shrimp diets ( p lt ; 0.05 ) ; the WSSV infection experimental group Litopenaeus vannamei cumulative mortality rate was 76.3% , the rate of immune protection 23.7% : astaxanthin impact on of Litopenaeus vannamei antioxidant enzyme gene transcription . Using beta - actin as an internal reference , by semi-quantitative PCR method to add astaxanthin as an antioxidant the the Litopenaeus vannamei liver pancreas MnSOD , CAT and GPX antioxidant enzyme gene transcription level . The results showed that dietary the 80mg/kg astaxanthin can significantly improve the antioxidant enzymes including SOD, CAT, GPX mRNA levels ( p lt ; 0.05 ) . Frequency of the status quo for the farmed shrimp diseases , the experimental Preliminary Study on Chinese shrimp aquaculture water of ( Fenaeus chinensis ) changes in the physical and chemical factors and shrimp white spot syndrome virus ( WSSV ) infection rates . To develop a pool of water quality monitoring and detection of WSSV OF found in WSSV infection rate higher in August , dissolved oxygen decreased rapidly , the temperature is the WSSV susceptible temperature range , the change of pH value , ammonium and nitrate a sudden increase in salt content . This shows that the deterioration of water environment can increase the susceptibility of the shrimp WSSV , or latent infection of WSSV shrimp stress-induced acute infection death

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquaculture technology > A variety of seafood, animal and plant breeding > Crustacean culture > Shrimp farming
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