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Expressions and Clinical Significance of Gastric Cancer Associated Markers CD133, CD324 in Gastric Cancer

Author: WeiMaoHua
Tutor: LiuZhong
School: Dalian Medical University
Course: Surgery
Keywords: Gastric cancer Cancer stem cells CD133 CD324 Immunohistochemistry
CLC: R735.2
Type: Master's thesis
Year: 2010
Downloads: 156
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Abstract


Background: Gastric cancer is one of the of the most common gastrointestinal malignancy, morbidity and mortality among the first of a variety of malignant tumors. The incidence of gastric cancer is a multifactorial regulation, multi-stage, multi-step, complex process of multiple genetic variants, molecular biology mechanism is very complex. Classic tumor cell origin theory that the tumor originated in the normal cells, tumor cells become mutated, and has an unlimited ability to replicate, was relatively endless growth of gastric cancer, the exploration and study of the development of mechanisms are mainly concentrated in the tumor-associated gene cloning and functional analysis, cell signaling and cell cycle regulation in several major areas. In recent years, the doctrine of tumors derived from stem cells and stem cell theory applied to cancer research, confirmed the self-renewal and differentiation ability of some tumor cells, and thus put forward the concept of \The theory put forward for oncology research has opened up new horizons. Currently think of cancer stem cells in tumorigenesis, metastasis and prognosis plays an important role of tumor stem cells related logo objects involved in tumor patients prognosis of analysis has a certain theoretical and clinical application prospects in our early experimental studies, found gastroepiploic the milky spots micro-metastases metastatic cells have cancer stem cell properties, the group of cells with high expression of CD133, CD324 expression decreased or negative expression of CD133 and CD324-may be one of the surface markers of gastric cancer stem cells. Objective: To detect gastric cancer related markers CD133 and CD324 expression in gastric cancer tissues, to analyze their relationship with clinicopathological factors of gastric cancer, and to explain the role and significance of CD133 and CD324 in gastric cancer invasion and metastasis. Comparative analysis of CD133 and CD324 expression in gastric cancer, and to explore the relationship between expression and prognosis of patients with gastric cancer associated markers CD133 and CD324. Methods: January 2000 - 2002 January Affiliated Hospital of Dalian Medical University, 100 cases of primary gastric carcinoma specimens were not done in all patients with preoperative radiotherapy, chemotherapy, with complete follow-up data; selected 10 cases of normal gastric tissue as control by SP immunohistochemical staining CD133 and CD324 expression. Using SPSS13.0 software was used for statistical analysis. Results: 1.CD133 negative expression in normal gastric tissue or only a small number of cells in the cell surface expression, the positive expression in gastric carcinoma, expression at the cell surface, the part associated with cytoplasmic expression, the positive rate of 57.0%. CD133 expression in gastric cancer tissues was significantly higher than normal stomach tissue, with a significant difference (P lt; 0.05). CD133 expression in gastric cancer tissues and patients' gender, age, tumor location, tumor size has nothing to do (P gt; 0.05). CD133 expression with tumor infiltration depth and higher (P lt; 0.05); increased with the decrease of the degree of tumor differentiation (P lt; 0.05); increased with the increase in the number of lymph node metastasis (P lt; 0.05); With The TNM staging increment higher (P lt; 0.01). CD133 expression of the five-year survival rate for gastric cancer after impact Univariate analysis showed that CD133-gastric cancer after five-year survival rate was significantly higher than CD133, there is a significant difference (P lt; 0.01); Multivariate analysis showed that, CD133 impact gastric cancer after a five-year survival rate factors, the regression coefficient is negative of CD133 expression in gastric cancer after five-year survival rate was negatively correlated. 2.CD324 showed strong positive expression in normal gastric tissue expression at the cell surface, some accompanied by cytoplasmic expression in gastric cancer tissues were negative or weakly positive, the positive rate of 41%. CD324 expression in gastric cancer tissues was significantly lower than that in normal gastric tissue, with a significant difference (P lt; 0.01). CD324 expression in gastric carcinoma patients' gender, age, tumor location, tumor size has nothing to do (P gt; 0.05). CD324 expression with tumor infiltration depth and lower (P lt; 0.01); decreases with the decrease of the degree of tumor differentiation (P lt; 0.05); decreased with an increase in the number of lymph node metastasis (P lt; 0.01); along with TNM stage incremental decrease (P lt; 0.01). CD324 expression in gastric cancer postoperative five-year survival rate impact Univariate analysis showed that CD324 gastric cancer after five-year survival rate was significantly higher than the CD324-, there is a significant difference (P lt; 0.01); Multivariate analysis showed that, CD324 impact gastric cancer after a five-year survival factors, the regression coefficient is positive CD324 expression in gastric cancer patients after five-year survival rate was positively correlated. 3.CD133, CD324 expression in gastric cancer tissues showed a significant negative correlation (r = -0.221, P lt; 0.05). CD133 CD324-co-expressed in patients with a worse prognosis. Conclusion: 1.CD133 and CD324 play a role in gastric carcinogenesis and the development process, has a certain reference value for the clinical evaluation of gastric cancer biological behavior and assessing prognosis of gastric cancer. 2.CD133, CD324 expression in gastric a negative correlation of CD133 CD324-co-expressed in patients with a worse prognosis, the assessment of the prognosis of patients with gastric cancer have a certain reference significance.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
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