Dissertation > Excellent graduate degree dissertation topics show

The Experimental Research for Pathologic Change of Nasal Mucosa in Allergic Rhinitis

Author: LiuJianGuo
Tutor: LiuYueHui
School: Nanchang University
Course: Department of Otolaryngology - Head and Neck Surgery
Keywords: allergic rhinitis eosinophile micrangium heme oxygenase-1 nasal mucosa remodeling
CLC: R765.21
Type: Master's thesis
Year: 2008
Downloads: 160
Quote: 1
Read: Download Dissertation

Abstract


[Objective] To establish the model of allergic rhinitis(AR) of Sprague-Dawley(SD) rats and observe the damage of nasal mucosa in morphology and the characteric of pathologic change in nasal mucosa of AR .[Methods] Total 180 healthy SD rats irrespective of female and male were randomly devided into the normal and AR groups it including intermittent allergic group and persistence group respectively . After establishing the model of allergic rhinitis by intraperitoneal injecting comphensive solution of ovalbumin(OVA)and AL(OH)3 then drope 5%OVA solution to nose. Sacrifice rats after 1、2、4、8、12、16weeks ,Apply hematoxylin- ereuth dyeing、make microvascular corrosion casts、transmission electrical microscope to analysis ultrastructure and apply immunohistochemistry technique(SABC method)、reverse transcriptio polymerase chain reaction technique to study the protein expressiong of HO-1、TGF-β1、FGF-2 in nasal mucosa AR.[Results] Compare to normal rats,intermittence group: 1)early 4 weeks after intensively sensitizating characteric of morph: endothelial cell edemata、desquamate,textus edemata in lamina epithelialis, goblet cells metaplasia、hypertrophy、productive secretion, angiectasis greatly in propria lamina, glandular organ hypertrophy, collagen oedemata in basal membrane, mucous layer thickening, chaotic textus strcture. Pseudostraified columnar ciliated epithelium or basal layer, with cilia loss and increasing inflammatory cell. eosinophils infiltrating and the expression of HO-1、TGF-β1、FGF-2 are difference significantly. 2) late8,12,16weeks:tissue edema significantly improved, mucosal cells tend to tidy and neatly arranged cilia, the hypertrophic goblet cells decreased significantly, the glands and expansive glandular tube tend to normal. infiltration of inflammatory cells in inherent layer reducing but not completely eliminated, fiber components increased at the same time. eosinophils infiltrating and the expression of HO-1、TGF-β1、 FGF-2 are gradually decreasing. Persistence group:1)early 4 weeks after intensively sensitizating characteric of morph : as well as intermittent group, mucosal injuried is very severe. but eosinophils infiltrating and the expres -sion of HO-1 are also not difference significantly compare to intermittence group(P>0.05)。2) late8,12,16 week:damaged epithelium repaired in a certain extent with fibration, but the restoration result of epithelial are untidiness, goblet cell volume is increased submucosal blood vessels increased, vascular wall thickening.especially in the mucous layer a large number of collagen deposited in basement membrane, gland volume increased、hyperplasia,glandular tube structure are fuzzy. further,EOS infiltrating and the expression of HO-1mRNA、TGF-β1、FGF-2 protein were significantly different compared to intermittence group(P <0.05). In the progress of remodeling the symptom of AR rats have the trend to reduce.[Conclusion] two groups of AR mucosa before 4 weeks there are mucosa damage,while after 4 weeks they are very different in pathologic change. compare to the intermittence group,the persistence group mucosa are remodeling to repair the damage of mucosa,it maybe the response of damage. Pathologiccharacteristic:repair after cell loss, subepithelial fibrosis, goblet cell proliferation, vascular proliferation, irregular thickening of the lumen, the gland volume increased, collagen deposition increasing in the basement membrane layer. While intermittence group rat mucosa remodeling aren’t significant, it maybe the recovery become more easy after deprivating allergen. In the progress of remodeling the nasal mucosa inflammation and oxidative damage have the trend to reduce .and TGF-β1、FGF-2 are participate in fibrosis repairing.

Related Dissertations

  1. The Reaserch of the Place Reconstruction of Old Industrial Building,TU984.114
  2. Protein phosphatase 2A Cα subunit knockout cardiac energy metabolism due to remodeling,Q78
  3. Heme Oxygenase is Involved in Salinity-Induced Tomato Lateral Root Development and Its Relationship with Hydrogen Peroxide,S641.2
  4. Cross-Talk between Ethylene and Heme-Heme Oxygenase Signal System in Primary Root Growth Inhibition of Etiolated Tomato Seedlings,S641.2
  5. The Involvement of Heme Oxygenase/Carbon Monoxide System in Cobalt Chloride-Induced Lateral Root Development in Tomato Seedlings,S641.2
  6. Salt-induced Vascular Remodeling and Telmisartan Intervention in Wistar Rats,R544.1
  7. Correlation of Humulus Pollen Allergic Rhinitis with HLA-DQA1 Gene,R765.21
  8. An Investigation of Allergens of Allergic Rhinitis and An Efficacy Analysis of 78 Cases Accepted Specific Immunotherapy in Dalian,R765.21
  9. Effects of High-salt Dieet on Blood Pressure and the Remodeling of Carotid Arteries and the Intervention of Telmisartan in Wistar Rats,R544.1
  10. Serum Levels of Insulin Like Growth Factor-1 and Cardiovascular Remodeling in Patients with Hypertension,R544.1
  11. A Stuty on the Diversification of Interleukin-17 of Asthma Mouse and the Affection of the Treament of Glucocorticoids,R562.25
  12. Analysis on the Prevalence of Allergic Rhinitis among 3～ 7 Years Old Children in Urumqi , S First-grade Kindergarten,R765.21
  13. p38MAPK on allergic rhinitis peripheral blood mononuclear cells IL-4, IL-5 effects,R765.21
  14. Combined surgery 34 cases of severe persistent allergic rhinitis efficacy,R765.9
  15. Effect of H2 O2 Pretreatment of Autologous Mesenchymal Stem Cells Transplantation on Ventricular Remodeling After Acute Myocardial Infarction,R542.22
  16. The Role of HA and CD 44 in the Airway Remodeling of Chronic Asthmatic Rat Model,R562.25
  17. Effects of Montelukast Plus Inhaled Budesonide on Airway Inflammation, Remodeling and the Expression of IL-33 Asthmatic SD Rats,R562.25
  18. Allergic rhinitis Chinese dialectical element in vivo and cytokine research,R276.1
  19. Role of Endogenous Carbon Monoxide in Whole Hepatic Ischemic Reperfusion Injury in Rats,R363
  20. Effects of Insulin on Left Ventricular Remodeling and Function in Rats with Myocardial Infarction,R542.22
  21. Effects of HMGB1 on Proliferation, Collagen Types Ⅰ and Ⅲ Mrna Expression in Cardiac Fibroblasts,R54

CLC: > Medicine, health > Otorhinolaryngology > Rhinology,nasal disease > Nasal disease > Rhinitis
© 2012 www.DissertationTopic.Net  Mobile