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Study on TFF3 and TR3 Expression in Gastric Carcinoma

Author: ChenMeiZuo
Tutor: RenJianLin；ZhangJing
School: Fujian Medical
Course: Internal Medicine
Keywords: Gastric cancer Trefoil factor 3 Orphan receptor TR3 Tissue microarray
CLC: R735.2
Type: Master's thesis
Year: 2008
Downloads: 108
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Abstract


Objective: To investigate the relationship TFF3 and TR3 with gastric development, transfer and related mechanism of action. Methods: (1) were extracted from gastric cancer SGC-7901 cells, GES immortalized normal gastric mucosal cell total RNA, reverse transcriptase respective cDNA to design synthetic TFF3 primers, the TFF3 mRNA by RT-PCR was used to detect in gastric cancer cell SGC-7901 cells (2) Western-blotting detection of gastric cancer cell line SGC-7901 gastric cancer tissues, GES immortalized normal gastric mucosa cell lines and normal gastric mucosa and expression in immortalized normal gastric mucosa cells in GES; TFF3 protein expression; (3) immunohistochemistry detection of SGC-7901 gastric cancer cells and paraffin sections of gastric cancer tissues TFF3 protein expression; (4) tissue microarray assay TFF3 protein and TR3 protein expression in 99 cases of postoperative gastric cancer tissues and 30 cases of normal gastric mucosa, statistical methods to analyze the relationship between TFF3 and TR3 with gastric cancer clinical and pathological staging and transfer, and further analysis of the correlation between the two. Results: (1) of TFF3 mRNA expression in SGC-7901 gastric cancer cell lines and gastric carcinoma higher amount, in GES immortalized cell lines of normal gastric mucosa, not detected in normal gastric mucosa. (2) Western-blotting method, TFF3 protein in gastric cancer cell SGC-7901, GES immortalized normal gastric mucosa cells in gastric carcinoma and normal gastric mucosa were not detected, the reason may be TFF3 molecular weight is too small, wild-type TFF3 small proportion of total protein in the cell, the experimental operation needs to be further improved and so on. (3) found by immunohistochemistry TFF3 high expression in gastric cancer SGC-7901 cells, TFF3 protein expression in paraffin sections of gastric cancer tissues compared with non-cancerous tissue was significantly increased, and the expression of cell differentiation; (4 ) tissue microarray results showed that gastric tissue TFF3 and TR3 positive expression rates were 77.8% and 68.7%, higher than both normal gastric tissue expression (26.7% and 30%), P <0.05: of TFF3, TR3 expression in gastric cancer clinical pathological stage and metastasis of the the TFF3 TR3 expression was positively correlated in different clinicopathological staging of gastric cancer tissues. Conclusions: (1) of TFF3 mRNA in gastric cancer SGC-7901 cells with high expression in GES immortalized normal gastric mucosa cells do not express; (2) Western-blotting detection TFF3 protein expression method of operation requires in-depth exploration; (3) of TFF3 protein expression in gastric carcinoma increased their expression in gastric cancer cell differentiation, well-differentiated gastric cancer tissues TFF3 protein expression lower expression of differentiated gastric carcinoma; (4) of TFF3, TR3 in gastric cancer expression and pathological staging of gastric cancer is closely related; (5) of TFF3 and TR3 expression in gastric carcinoma has a good piece.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
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