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Objective: To investigate recombinant human interleukin- 2 ( IL - 2 ) and alpha - interferon ( alpha - IFN ) in human lung adenocarcinoma cell A549/CDDP multidrug resistance (MDR) reversal effect and its mechanism . Methods : MTT assay IL-2, α-IFN and IL - 2 before and after the alpha - IFN processing A549/CDDP cell sensitivity to drug CDDP . The fluorescence spectrophotometric determination of IL - 2 and alpha - IFN and IL - 2 alpha - IFN treatment before and after A549/CDDP cells rhodamine amount of aggregates . Flow cytometry processing before and after A549/CDDP cells the expression of P-glycoprotein ( P - gp ) . Results : 1 by IL-2 alpha - IFN and IL-2 alpha - IFN processing the chemotherapy drugs of CDDP A549/CDDP cells IC 50 decreased with time , improve the sensitivity of the drug , compared with the control group , the difference was significant ( P < 0.05 ) . 2 by IL - 2 , alpha - IFN and IL-2 α-IFN for 48 hours after , A549/CDDP cell rhodamine mean fluorescence intensity , respectively : IL-2 as 4.82 , alpha - IFN for the 4.25 , IL- 2 alpha - IFN to 9.13 , while the untreated control group was 2.38 , compared with the control group , the difference was significant ( P < 0.05 ) . By IL - 2 , alpha - IFN and IL - 2 alpha -IFN for 48 hours after , A549/CDDP cells mean fluorescence intensity of P-gp , respectively 5.98,8.90,3.03 , while the untreated control group as compared to 15.23 compared with the control , the differences were statistically significant ( P < 0.05 ) . Conclusion : IL - 2 , alpha - IFN and IL-2 α-IFN increase A549/CDDP cell sensitivity to CDDP possible mechanism for the inhibition of P-gp expression , the ultimate reversal of multidrug resistance of tumor cells .
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