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Objective: 56 cases of gastric cancer expression of CD24 and CD34 detection probe CD24, CD34 protein expression in gastric cancer occurrence, development, histological grade, clinical stage, invasion, metastasis and angiogenesis of gastric cancer. To analyze CD24 in gastric carcinogenesis, development and prognosis significance for gastric cancer early diagnosis, treatment and prognosis prediction provides a theoretical basis. Methods: Immunohistochemical SP method detected 56 cases of surgical resection and confirmed by pathological examination of gastric specimens CD24 and CD34 expression. The positive rate of CD24 expression to represent, CD34 expression with microvessel density (MVD) representation. Whole group of 56 patients with gastric cancer were followed up for CD24 protein expression and prognosis of patients with gastric cancer survival analysis. Results: 1. CD24, CD34 in gastric carcinoma: CD24 staining mostly localized in the cytoplasm and cell membrane. In 56 cases of gastric carcinoma, 45 cases (80.36%) CD24 protein expression. CD34 is mainly expressed in vascular endothelial cell membrane. Microvascular stained brown, uneven distribution showed heterogeneity, the most densely stained areas or \2. CD24, CD34 and gastric cancer clinical features: In the eight cases of early gastric cancer, there are three cases of CD24 was weakly positive and 48 cases of advanced gastric cancer, 38 cases were strongly positive or moderately positive, the difference was significant statistical significance (P lt; 0.0001). The histological type of gastric cancer in well-differentiated, moderately differentiated, poorly differentiated tumor cells with CD24 expression was no significant correlation (r = 0.1901, P = 0.1604). TNM staging with high CD24 expression was statistically significant (χ2 = 18.4029, P = 0.0004), TNM staging Ⅱ, Ⅲ, Ⅳ and Ⅰ, the difference was statistically significant (P values ??were 0.0010,0.0009,0.0003) . Gastric cancer cell infiltration in T2, T3, T4 and T1, the difference was statistically significant (P values ??were 0.0047,0.0008,0.0005). Lymph node metastasis N1, N2, N3 and N0, the difference was statistically significant (P values ??were 0.0027,0.0004, lt; 0.0001). MVD value of histological grade and lymph node metastasis (P gt; 0.05), while the depth of tumor invasion and TNM clinical stage (P lt; 0.05) 3.CD24 and MVD and prognosis: 56 cases of gastric cancer MVD field of view at 400 times the mean of 44.00 ± 11.08. With the enhancement of CD24 positive cells, MVD also increased, both of which were positively correlated (r = 0.72, P lt; 0.0001). This shows that, CD24 expression may promote the growth of tumor blood vessels closely. High expression of CD24 protein in patients with a median survival was significantly reduced, and other groups, the difference was statistically significant (P = 0.0032). Conclusions: 1. CD24 protein expression in gastric cancer cell invasion, lymph node metastasis and TNM stage number were positively correlated, suggesting that CD24 protein in tumor occurrence and development plays an important role. 2. MVD value of histological grade and lymph node metastasis, but not with the depth of invasion and TNM swollen clinical stage. 3. CD24 protein expression and angiogenesis in gastric cancer, gastric cancer cell proliferation was positively correlated, suggesting that CD24 may be a way to promote the growth of tumor blood vessels, CD24 protein expression in gastric cancer patients with high median survival was significantly reduced, as a sign of gastric cancer invasion and metastasis and prognostic indicators.
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