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transplant possible. In this study, the pre-denatured rabbit sciatic nerve tissue cells transplanted into microencapsulated SD rats after SCI observed at the spinal cord hemisection after implantation of microencapsulated xenogeneic host sciatic nerve tissue cells in peripheral blood T lymphocytes group influence, and explore sciatic nerve tissue microencapsulated xenogeneic cell transplantation for treatment of spinal cord injury repair possible mechanisms. Methods Healthy adult rabbits 8; healthy adult Sprague-Dawley (SD) rats were 88, SD rats were randomly divided into three groups: A (microencapsulated sciatic nerve tissue transplantation group, n = 40); B group (tissue cell suspension transplantation group, n = 40); C group (control, n = 8). After a good group of rats in each group underwent right hind limb motor function BBB score. Denaturation take adult rabbits treated with bilateral sciatic nerve tissue made of cell suspension, low-speed centrifugation and 1.5% sodium alginate solution was mixed and sprayed into 20 mmol / L of barium chloride solution made microencapsulated sciatic nerve tissue cell suspension. A, B group the right half of the rat spinal cord injury model T10, and were implanted in the lesion adsorption 10μl of cell suspension of microencapsulated gelatin sponge tissue and tissue cell suspensions of gelatin sponge, C group no treatment . A group and B group were randomly selected six rats, respectively, after the first 1,3,7,14,28 d on the right hind limb motor function BBB score. Group A and group B, respectively, after the first 1,3,7,14,28 days each time randomly selected eight rats tail blood, and the other group C 8 rats also OK tails blood surgery. Flow cytometry of T lymphocyte subsets CD4, CD8-positive cells. The results of spinal cord injury (SCI), the cell suspension group CD4 ~ T cell counts in the first three days than normal control group had elevated (P lt; 0.05), 7, 14 and 28 days, the difference was significant (P lt; 0.01); This group CD8 ~ T cell counts at day 7, 14 and 28 are different than the normal control group (P lt; 0.05). The microcapsule group at each time CD4 ~ T cells and CD8 ~ T cells compared with normal control group had no difference (P gt; 0.05). 7, 14 and 28 days, the cell suspension group CD4 ~ T cells was significantly higher than the microcapsule group (P lt; 0.01), the group CD8 ~ T cell counts at 7, 14 and 28 days, compared with micro- sac group increased. Each group the right hind limb motor function preoperatively BBB scores were 21 points, SCI were lower after 3 and 7 days and no significant difference between the groups; 14th day of group A rat right hind limb functional recovery was better than B, Group C (P lt; 0.05); 28 days in group A rat right hind BBB scores than B, C group rat right hind BBB score difference was significant (P lt; 0.01). After SCI, the rats right hind limb motor function in varying degrees of recovery, but recovery is best mc group. Conclusion sciatic nerve tissue transplantation of microencapsulated xenogeneic can effectively prevent rat CD4 ~ T cells and CD8 ~ T cell activation and value-added, and promote hindlimb motor function recovery.
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