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In this experiment, screened four strains of Monascus Monascus sp . ZZ, Monascus rubber ZK, Monascuspurpurers GX, Monascus sp. XM, to obtain high-yielding strains of ergosterol - ZZ , containing per gram of bacterial cells the ergosterol 675.4μg, and its ergosterol production , 120h peak strain -ZZ ergosterol production , per gram of bacteria to contain 746.1μg ergosterol . By HPLC and UV measurement methods, there is no big difference between the two . Liquid culture conditions , inoculum size of 10% per gram of bacteria with 772.8μg ergosterol , the greater the vaccination greater the amount of biomass ; initial pH of the bacterial biomass have a certain influence , but ergosterol content is low ; culture temperature was 30 ℃, per gram of bacteria containing 747.6μg ergosterol , the temperature is too high to be interested in the synthesis of ergosterol produce inhibition ; speed 220r/min per gram of bacteria containing 775.1μg ergosterol , speed too cause cell damage and thus reduce biomass , the speed is too small and would adversely ergosterol synthesis . Medium optimize our first single factor screening get : 3.0% of lactose per gram of bacteria ergosterol 1024.1μg , beef extract 2.0% per gram of bacteria ergosterol The content 1351.1μg the MgSO 4 < / sub > per gram of bacteria 1.00 % when the ergosterol content of 957.9μg . Select filtered out single factor , considering the the ergosterol yield and cost , the five factors finalization of glucose , yeast extract , ( NH 4 < / sub > ) 2 < / sub> SO 4 , MgSO 4 , KH 2 PO 4 regression analysis with D- priority the ε analysis and secondary saturated D- optimal design method of trial design and establishment of polynomial regression model , the highest indicators by finishing polynomial combination of factors : glucose 3.7% , yeast extract 3.0% , ( NH 4 ) 2 SO 4 1.7%, MgSO 4 0.5%, KH 2 PO 4 1.6%, and the combination of verification, have per gram of bacteria ergosterol average 2678.6μg and polynomial regression analysis of the highest indicators close .
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