|
Objective: patients with type 2 diabetes and fasting plasma glucose screening injury patients in Urumqi, China second poor and needy hospitals and communities and Kashgar East Road, Urumqi, community health service stations around the surrounding communities eligible patients for a week metformin. Focused on Uighur patients encoding organic cation transporter 1 the OCT1 gene R61C sites, the G465R sites 420del loci polymorphism detection, to identify them with metformin efficacy. Attempt to explore the relationship between gene variants and metformin efficacy, and to find with metformin efficacy may be related to the genotype and genotype combinations, to provide a reference for metformin in the development of individualized treatment. Method: In the second poor and needy hospitals in Urumqi, China, and the surrounding communities and Kashgar East Road, Urumqi, community health service stations surrounding communities screening for drug intervention in patients with type 2 diabetes and impaired fasting glucose injury patients. Daily 2000mg of metformin intervention of included subjects a week. Fasting blood glucose, lipids collected before and after drug intervention. DNA extraction and PCR-RFLP technique, patients on the drug intervention the OCT1 gene R61C sites, the G465R sites, 420del locus genotyping, genotyping and efficacy of metformin t test. Results: After a week of drug intervention, subjects FPG and LDL two indicators, significant before and after treatment. In the OCT1 gene R61C sites, carrying the TT genotype, the CT genotype two kinds variant subjects carrying the CC wild genotype subjects before treatment and after treatment indicators (including FPG, Chol TG, LDL, HDL) the comparison of the indicators change was not statistically significant (P gt; 0.05). Carrying the GA genotype the OCT1 gene G465R sites, the two variants of the AA genotype subjects and carry the GG the wild genotype the subjects before treatment and after treatment indicators (including FPG, Chol , TG, LDL, HDL) the relatively less significant (P gt; 0.05), but comparison of the changes in the indicators found on LDL change was statistically significant (P = 0.05). In the OCT1 gene 420del point to carry Pdel of Tdel two variants subjects carrying the ATG wild genotype subjects before treatment and after treatment indicators (including FPG, Chol, TG, LDL not statistically significant (P gt; 0.05), HDL) comparison, but comparison of the changes in the indicators on TG changes were statistically significant (P = 0.03 lt; 0.05). OCT1 gene carrying at least one mutation sites (including the R61C, G465R and 420del sites) genotype subjects (14) and three loci are wild genotype subjects (6 ) before treatment and after treatment indicators (including FPG, Chol, TG, LDL, HDL) comparison of the indicators change was not statistically significant (P gt; 0.05). Conclusion: The study data show that the the R61C the G465R and 420del-site mutation in the the OCT1 gene on the impact of metformin on blood glucose regulation effect of the Xinjiang Uygur type 2 diabetes and fasting plasma glucose injury patients, but may affect metformin on type 2 diabetes in the Xinjiang Uygur and fasting blood sugar control damage in patients with hyperlipidemia.
|