Dissertation > Excellent graduate degree dissertation topics show

Effect of Smoking on the Expressional Levels of Matrix Metalloproteinase-9 and Its Inhabitor-1 in Sensitized Rats

Author: WangRuiYing
Tutor: DuYongCheng
School: Shanxi Medical
Course: Department of Respiratory Medicine
Keywords: Asthma Matrix metalloproteinase Tissue inhibitor of metalloproteinase Smoke
CLC: R562.25
Type: Master's thesis
Year: 2008
Downloads: 41
Quote: 0
Read: Download Dissertation

Abstract


Objective: To observe the smoking sensitized rat lung tissue matrix factor matrix metalloproteinase-9 and metalloproteinase tissue inhibitor 1 expression, and to explore the role of smoking in airway remodeling in asthma. : 24 clean male Wistar rats by random number table will be divided into a control group, sensitized group and smoking sensitized group, n = 8. The two groups after application of ovalbumin (OVA) sensitization and inhalation challenge, rat asthma model; inspire three weeks will be 30 minutes each time atomization smoking sensitized rats placed homemade smoked inside passive smoking, once a day, every 10 cigarettes, each burning cigarette 1 hour, 6 days a week, smoking a total of eight weeks. Detected by immunohistochemical method rat airway epithelial cell matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase 1 (TIMP-1) protein, the simultaneous measurement of the thickness of the bronchial wall; reverse transcription - polymerase MMP-9mRNA, TIMP-1mRNA chain reaction (RT-PCR) to detect the lung tissue content. Results: 1. Smoking sensitized group airway wall thickness (23.28 ± 2.38) was significantly higher than the sensitized group (20.06 ± 2.94) and control group (11.64 ± 2.43), the difference was statistically significant (P lt; 0.05); sensitized group higher, the difference was statistically significant (P lt; 0.05). 2 smoking sensitized group lung tissue expression of MMP-9mRNA airway epithelial cells in MMP-9 protein content (32.78 ± 2.60) (0.49 ± 0.02) was significantly higher than the sensitized group (0.41 ± 0.04; 23.05 ± 2.11) and control group (0.23 ± 0.03; 15.88 ± 1.69), the difference was statistically significant (P lt; 0.01); the sensitized group higher than that in the control group, the difference was statistically significant (P lt; 0.01). Smoking sensitized group lung tissue expression of TIMP-1mRNA airway epithelial cells in TIMP-1 protein content (34.54 ± 2.9) were higher than the sensitized group (0.37 ± 0.05 (0.53 ± 0.02); 21.25 ± 2.28) and control group (0.23 ± 0.04; 15.78 ± 1.97), the difference was statistically significant (P lt; 0.01); the sensitized group higher than that in the control group, the difference was statistically significant (P lt; 0.01). . Lung tissue MMP-9/TIMP-1mRNA ratio: smoking sensitized group (0.91 ± 0.05) is lower than the sensitized group (1.12 ± 0.06) and control group (1.03 ± 0.09), the difference was statistically significant (P lt ; 0.01); the sensitized group higher than that in the control group, the difference was statistically significant (P lt; 0.01). Airway epithelial cells MMP-9/TIMP-1 protein content ratio: Smoking sensitized group (0.94 ± 0.03) lower than the sensitized group (1.09 ± 0.07) and control group (1.01 ± 0.06), the difference was statistically significant respectively (P lt; 0.05, lt; 0.01); the sensitized group higher than that in the control group, the difference was statistically significant (P lt; 0.01). Conclusion: Smoking sensitized rat lung tissue can be MMP-9 and TIMP-1 overexpression, imbalance and increased airway remodeling.

Related Dissertations

  1. Distribution of the Contents of Pectin and Cellulose and Their Effect on Smoking Quality in Flue-cured Tobacco of China,S572
  2. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  3. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  4. Glucocorticoid treatment of bronchial asthma patients serum IL-18, IL-33 levels in,R562.25
  5. The Role of HA and CD 44 in the Airway Remodeling of Chronic Asthmatic Rat Model,R562.25
  6. CFD Simulation Study on Environmental Control System of a Subway Station Platform in Wuhan,TU831.8
  7. The Effect of Unilateral Adrenalectomy on Transfor-mation of Adrenal Medullary Chromaffin Cell in Asthmatic Ratsand the Interference of Kidney-Tonifying Recipe,R562.25
  8. Expressions of Matrix Metalloproteinase-3 and Matrix Metalloproteinase-9 in the Serum and Sacroiliac Joint of Spondyloarthropathy,R593.2
  9. The Influence in AMCC’s Structure and Function by Serum of Asthmatic Rats and the Intervene Effect of Kidney-tonifying Recipe,R285.5
  10. Combine Traditional Chinese and Western Medicine Treatment Cough Variant Asthma Cilnical Observation,R562.25
  11. The Clinical Significance of the Test for the hs-CRP and SP-D in the Serum to the Patient of AECOPD,R563.9
  12. Effect of p38MAPK on Expression of MMP-9 and TIMP-1 Induced by High Glucose in Glumeruli Mesangial Cells,R587.2
  13. A Study on the Relationship between CD4~+ CD25~+ T Regulatory Cell and Immune Tolerance in Lymph in Bronchial Asthmatic Rat,R562.25
  14. Inhibiting activation of PBMCs Dex and TP expression IL-4, IL-5 and IL-13 Mechanism,R562.25
  15. Experimental Study on the Impacts of Celecoxib on Rat Lumbar Intervertebral Degeneration Disc Nucleus Pulposus,R681.53
  16. Ganglioside GD1a on MMP-9 in different cell types in the regulation of,R96
  17. Studies on Synthesis and Anti-inflammatory Activities of Imidazolidine-2, 4-dione and Its Derivatives,R96
  18. Preparation and Application of Zinc Stannate Coated Inorganic Nano-Particles as Flame Retardant,TB383.1
  19. Of Guben Zhikepingchuan particles pharmacodynamic study,R285.5
  20. DM6437 based Video Smoke Detection System,TP391.41
  21. Based on image processing room video fire smoke recognition method,TP391.41

CLC: > Medicine, health > Internal Medicine > Respiratory system and chest diseases > Trachea and bronchial disease > Bronchial disease > Bronchial asthma
© 2012 www.DissertationTopic.Net  Mobile