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The purpose of observation tanshinone Ⅱ A (Tan, Ⅱ A), astragalus polysaccharide (APS) and its compatibility hypertension in patients with serum damage the umbilical vein endothelial cell strains (ECV-304) activity, morphology, endocrine function and intracellular free calcium concentration affect. Explore research the Chinese monomer and its compatibility Yiqihuoxue protective effect on endothelial cells, so as to provide experimental basis Yiqihuoxue Chinese combat hypertension protect endothelial cells. The methods were divided into five groups: control group, model group, Tan Ⅱ A group, APS Group, compatibility group. Methyl thiazolyl tetrazolium bromide (MTT) assay the activity of the cells, cell morphology was observed under inverted phase contrast microscope and HE staining, nitrate reductase assay cells secrete nitric oxide (NO) concentration, non-equilibrium method for the determination of cell secretion concentration of endothelin (ET), laser scanning confocal microscope to detect the concentration of intracellular free calcium. Results Serum role 24h cell activity of model group compared with the control group (P <0.05); When Tan Ⅱ A (40,20,10 μg / ml), APS (640,320,16 μg / ml) group of effector cells, cell higher activity than the model group (P <0.05 or P <0.01); When Tan, Ⅱ A and APS of compatibility group the role of cells, cell activity compared with the model group high (P <0.01), but also than Tan Ⅱ A or APS alone to be high (P < 0.05); 2 cell comparison between the model group and the control group, significant morphological differences between the cells; drug group cell morphology with increased drug concentration and the closer to the normal cell morphology; 3 NO secretion model group than the control group ( P <0.01); model group secretion of ET higher than those in the control group (P <0.01); Tan, Ⅱ A, the APS group and compatibility group secretion of NO compared with the model group, high (P <0.05 or P <0.01), Tan, Ⅱ A, the APS group and compatibility Group secretion of ET compared with the model group (P <0.05 or P <0.01); Tan Ⅱ A, secreted by the ASP group NO, ET and corresponds to the compatibility group, a significant difference (P <0.05); 4. model group and the control group In comparison, the fluorescence intensity (P <0.01), Tan Ⅱ A, APS and its compatibility group with model group, the fluorescence intensity of small (P <0.05), and compatibility group than simply with Tan Ⅱ A or APS of fluorescence intensity to be small (P <0.05 ). Conclusion 1. Cells of model group decreased activity, morphological changes and functional damage; 2.Tan Ⅱ A, APS and its compatibility can inhibit cell activity reduce, repair, change the cell morphology and the protection of the function of the damaged cells; Tan Ⅱ A, APS The compatibility of the protective effect is better than the separate effects of Tan Ⅱ A or APS.
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